Fabrication and Characterization of Chelate-Setting β-Tricalcium Phosphate Cements with Enhanced Bioresorbability

Abstract:

A novel chelate-setting β-tricalcium phosphate (β-TCP) cement with anti-washout properties have been fabricated previously. This cement has been set on the basis of chelating ability of inositol phosphate (IP6). In this study, the ball-milling and surface-modification conditions of starting β-TCP cement powders were optimized in terms of bioresorbability. Starting powders were prepared by simultaneously ball-milling at 300 rpm for 3 h with 1 mm diameter ZrO₂ beads and surface-modifying with 40 cm³ of 3000 ppm IP6 solution. The resulting starting powder was consisted of β-TCP single phase, and had high specific surface area of 48.3 m²∙g^-1. Cement pastes were prepared by mixing the starting powder and the aqueous solution composed of 2.5 mass% sodium hydrogen phosphate, 1.5 mass% citric acid and 1.0 mass% sodium alginate at a powder/liquid ratio of 1/0.90 [g∙cm^-3] for 2 min. After setting in pure water for 72 h, compressive strength of the cement specimens was higher than that of human cancellous bone. Dissolution rate of Ca²⁺ ions was measured by according to Japanese Industrial Standard T 0330-3. The results of Ca²⁺ ions dissolution rate test demonstrated that the cement specimens derived from the above starting powder were the highest dissolution rate among examined ones. This cement would be expected as bone fillers with high bioresobability.

• Periodical: Key Engineering Materials (Volume 720)
• Pages: 157-161
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.720.157
• Online since: November 2016
• Authors: Kohei Nagata, Toshiisa Konishi, Michiyo Honda, Mamoru Aizawa*
• Keywords: Bioresorbability, Calcium-Phosphate Cement, Chelate-Setting Cement, β-Tricalcium Phosphate

References

[1] S. Takagi, L.C. Chow, K. Ishikawa, Formation of hydroxyapatite in new calcium phosphate cements, Biomaterials 19 (1998) 1593-1599.

DOI: 10.1016/s0142-9612(97)00119-1

[2] X. Zhonglin, H. Zhang, A. Jin, Y. Jiandong, R. Li, A. Jun, F. Wei, L. Xiaoyong, Correlation between degradation and compressive strength of an injectable macroporous calcium phosphate cement, J. Alloy. Compd. 520 (2012) 220-225.

DOI: 10.1016/j.jallcom.2012.01.022

[3] A. Bigi, I. Cantelli, S. Panzavolta, K. Rubini, α-Tricalcium phosphate-gelatin composite cements, J. Appl. Biomater. Biom. 2 (2004) 81-87.

[4] W. Tingting, S. Haishan, Y. Jiandong, Effect of PLGA/lecithin hybrid microspheres and β- tricalcium phosphate granules on the physicochemical properties, in vitro degradation and biocompatibility of calcium phosphate cement, Royal Society of Chemistry 5 (2015).

DOI: 10.1039/c5ra06861d

[5] K. Kiminami, K. Matsuoka, K. Nagata, T. Konishi, M. Honda, G. Hayashida, K. Nakano, M. Nagaya, H. Arimura, H. Nagashima, M. Aizawa, Development of bioresorbable calcium-phosphate cements hybridized with gelatin particles and their in vivo evaluation using pig's tibia model, Key Eng. Mater. 631 (2015).

DOI: 10.4028/www.scientific.net/kem.631.397

[6] T. Konishi, S. Takahashi, Z. Zhuang, K. Nagata, M. Mizumoto, M. Honda, Y. Takeuchi, H. Nagashima, M. Aizawa, Biodegradable β-tricalcium phosphate cement with anti-washout property based on chelate-setting mechanism of inositol phosphate, J. Mater. Sci.: Mater. Med. 24 (2013).

DOI: 10.1007/s10856-013-4903-8

[7] T.H. Dao, Polyvalent cation effects on myo-inositol hexakis dihydrogen phosphate enzymatic dephosphorylation in dairy waste water, J. Environ. Qual. 32 (2003) 694-701.

DOI: 10.2134/jeq2003.6940

[8] C.J. Martin, W.J. Evans, Phytic acid-metal ion interractions. II. The effect of pH on Ca(II) binding, J. Inorg. Biochem., 27 (1986) 17-30.

DOI: 10.1016/0162-0134(86)80105-2

[9] M. Aizawa, T. Konishi, M. Honda, M. Matsumoto, Relationship between bioresorption and solubility in vitro of chelate-setting calcium-phosphate cements - verification on the basis of JIS T 0330-3 Bioceramics-Part 3: Testing method of measuring dissolution rate of calcium phosphate ceramics, - J. Soc. Inorg. Mater. Jpn., 22 (2015).

DOI: 10.4028/www.scientific.net/kem.758.194

[10] T. Konishi, M. Honda, T. Yoshioka, S. Hayakawa, M. Aizawa, Preparation of α-tricalcium phosphate powders surface-modified with inositol phosphate for cement fabrication, Key Eng. Mater. 631 (2015) 113-118.

DOI: 10.4028/www.scientific.net/kem.631.113

[11] Y. Öztürkmen, M. Canıklıoğlu, M. Karamehmetoğlu, E. Şükür, Calcium phosphate cement augmentation in the treatment of depressed tibial plateau fractures with open reduction and internal fixation, Acta. Orthop. Traumatol. Ture. 44 (2010) 262-269.

DOI: 10.3944/aott.2010.2406

[12] E. Fernández, M.P. Ginebra, M.G. Boltong, R.M.H. Verbeeck, J.A. Planell, Kinetic study of the setting reaction of a calcium phosphate bone cement, J. Biomed. Mater. Res. 32 (1996) 367-374.

DOI: 10.1002/(sici)1097-4636(199611)32:3<367::aid-jbm9>3.0.co;2-q

[13] H. Manabe, T. Konishi, M. Mizumoto, M. Honda, M. Aizawa, In vitro bioresorbability of chelate-setting cements with various calcium-phosphate phases, phosphorous Res. Bull., 26 (2012) 105-108.

DOI: 10.3363/prb.26.105