Spectroscopic Studies of Adsorbed Myoglobin on Zinc Containing Hydroxyapatite

Abstract:

The knowledge on human tissue is very important to recognize desirable properties of biomaterials. Host cells, extracellular matrix, integrated vessels and interstitial fluids create a complex and dynamic system able to regenerate and respond to environmental stimuli. Myoglobin is a protein with most of α-helices on its secondary structure, and responsible for oxygen binding and release in muscles, by the heme group. This work investigates the Mb adsorption process onto zinc-hydroxyapatite (ZnHA) surface by spectroscopic studies. To do so, ZnHA (0.05 g) was incubated with 4mL of 2mg Mb/mL on phosphate buffer solution pH 6.0 for 24h at 37°C. The FTIR analyses of ZnHA powders before and after protein adsorption provided information concerning the protein content. UV-Vis spectrocopy in the reflectance mode suggested a mixture of MbO₂ and Met Mb on lyophilized solid Mb, and the prevalence of MetMb form when Mb was adsorbed on ZnHA sample. The decrease of UV-Vis secondary bands suggests interactions through the Mb heme group and the ZnHA surfaces. Circular Dichroism (CD) spectroscopy indicated the maintenance of the Mb α-helices secondary structure after the adsorption process on ZnHA powders.

• Periodical: Key Engineering Materials (Volume 720)
• Pages: 41-45
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.720.41
• Online since: November 2016
• Authors: Andrea Machado Costa, Elena Mavropoulos, Alexandre Malta Rossi, Marcelo Henrique Prado da Silva*
• Keywords: Hydroxyapatite, Myoglobin, Spectroscopy, Zinc

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