The HL-60 Cells Response to Various Particle Sizes of Tetracalcium Phosphate Ceramic Delivery System

Abstract:

The specific objective of this investigation is to study the effect of tetracalcium phosphate (TeCP) delivery system fabricated from three different particle sizes (<38, 45 and 75 um) on the viability of HL-60 cell line in culture. Each TeCP reservoir was fabricated using standard protocols. The sintered microcrystal material were cold pressed at 5000 kg compression load, sterilized and loaded into wells pre-plated with 10⁶ HL-60 cells. At the end of 24, 48, and 72 hours, the supernatants (cell suspension) from the wells were collected for cell count (a hemacytometer method) and lactate dehydrogenase (LDH) assays. The cells were evaluated for structural cellular damage by using conventional histopathological protocols (H&E stain). Results obtained from this investigation suggest the followings: (i) particle sizes are key elements in developing TeCP delivery system, (ii) the use of <38 um particle sizes would be favorable for long duration of delivering biologicals from the TeCP reservoirs due to the viability of HL-60 cells, (iii) LDH activities were directly proportional the particle sizes of the ceramic capsules compared to the control and (iv) there were less cellular structural changes in devices fabricated from <38 um particle sizes and the cells became more reactive with an increase in particle sizes.

• Periodical: Key Engineering Materials (Volume 720)
• Pages: 77-81
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.720.77
• Online since: November 2016
• Authors: Hamed Benghuzzi*, Michelle Tucci
• Keywords: Biomaterial, Ceramics, Drug Delivery System, Particle Sizes of Ceramics

References

[1] Benghuzzi, HA, England, BG and Bajpai, PK. Characterization of Ceramic Drug Delivery Devices: The Effect of Various Temperature of Incubation on the Delivery of Biological, Biomed Sci Instrum (ISA, Paper #92-0131). 1992; 28: 129-134.

[2] Apple*, MJ, Benghuzzi, HA and Parsell, D. Interrelationship Between Buffer System and Particle Sizes of TCPL Drug Delivery System. Biomed Sci Instrum (ISA). 1996, Paper #96-007, 1996; 32: 47-54.

[3] Benghuzzi H, Puckett A, Robert B, and Tucci M: Modification of ALCAP, TCP, and HA Surfaces by Isolated Mice Osteoclasts in Culture. Biomed Sci Insrum, (Paper #99-055 ISA, 0227-7576/97/321), 1999; 35: 321-326. [PubMed].

[4] Benghuzzi H, Puckett A, Tucci M, Roberts B. Bioceramics surface modification by means of osteoclasts in culture. Biomed Sci Instrum. 1999; 35: 321-6.

[5] Benghuzzi H, Tucci MA, Ibrahim J; Effects Of Polylactic Acid Coating and Compression Load on the Delivery of Protein and Steroid from HA Ceramic Devices. Biomed Sci Instrum. 2015 Apr 10; 51: 122-127.

[6] Benghuzzi, HA. and Bajpai, PK. Effects of Compression Loads on Density and Controlled Release of High and Low Molecular Weight Compounds from ALCAP Ceramic Implants. Digest of Papers, Seventh Southern Biomedical Engineering Conference. Greenville, SC. October 27-28, 1988. McGregor and Werner, Washington, DC., D.D. Moyle (Editor), 1988; 32-35.

[7] Benghuzzi, HA and Bajpai, PK. A Ceramic System for Delivering Mixtures of Steroids. Proceedings of the 14th North East Bioengineering Conference, Boston, MA. March 27-28, 1989. IEEE., New York, NY., 1989; 61-62.

[8] Jump up to: a b Gallagher R, Collins S, Trujillo J, et al. (1979). Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia,. Blood 54 (3): 713–733. PMID 288488.

DOI: 10.1182/blood-2016-10-748780

[9] Jump up Breitman, T, S. Collins, B. Keene, (1980).