Potential of a Novel Thermotolerant Lipase Bacillus stearothermophilus nr22 (Lip.nr-22) as Additive in High Temperature Operated-Neutral pH Liquid Detergent

Abstract:

Enzyme-added detergent must have the capability to operate at high temperature to support the enzyme proteins to clean soiled-fabrics at optimum conditions. Lipase from Bacillus stearothermophilus nr22 (Lip.nr-22) has improved the oil removal from soiled-cotton fabric by 38.8-51.4% in 4 types of local commercial detergents. The later was the oil removal from an unrevealed detergent. The optimum conditions were 108U/ml Lip.nr-22 in 0.1M, pH 7.0, washing temperature and washing time interval as 80°C and 40 min, respectively; shaking wash at 300 rpm and percentage of detergent concentration as 0.5. Lip.nr-22 is a very potential enzyme in high temperature-neutral pH operated laundry detergent formulations. It has exhibited a very excellent thermostability at 80°C, was very stable with surfactants, commercial detergents as well as with oxidizing agents (H₂O₂, NaBO₃H₂O and NaClO). Lip.nr-22 as additive in detergent formulation is a promise for better detergent formulation.

• Periodical: Key Engineering Materials (Volume 735)
• Pages: 136-142
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.735.136
• Online since: May 2017
• Authors: Nik Raikhan Nik Him*, Nurul Shafika Azmi
• Keywords: Bacillus stearothermophilus nr22, Microbial Lipase, Neutral pH Detergent, Temperature Stability, Washing Temperature

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