Enhancement of Hydrogen Production from Sugarcane Bagasse by Klebsiella pneumoniae subsp. pneumonia DSM 30104(T) Using Statistical Methods

Abstract:

This study employed statistically based experimental designs to optimize environmental factors for H₂ production from sugarcane bagasse hydrolysate by K. pneumoniae subsp. Pneumonia DSM 30104(T) isolated from wastewater sludge using statistical method. The 12 runs of Plackett-Burman design were used to classify important factors influencing the H₂ production from sugarcane bagasse hydrolysate by K. pneumonia subsp. pneumonia DSM 30104(T). Mutual interaction between the significant factor and their optimal values that brought the maximum H₂ production (mL H₂/L) were further investigated using Box-Behnken design of response surface method. Experimental results indicated that yeast extract, ammonium chloride, potassium chloride, calcium chloride, iron (II) sulfate and total sugar of sugarcane bagasse had an interdependent effect on the maximum H₂ production while only interaction effect between yeast extract and calcium chloride had statistically significant (P≤0.05) influences on the maximum H₂ production. Optimal conditions for the predicted maximal H₂ production were 7.50 g/L yeast extract, 0.50 g/L ammonium chloride, 15.0 g/L potassium chloride, 0.75 g/L calcium chloride, and 10.0 g/L total sugar. At the optimal condition, the maximum H₂ production of 277 mL H₂/L was estimated from Box-Behnken design that more than 9 times compared to Plackett-Burman design. The highest ratio of butyric acid to acetic acid (B/A ratio) of 1.47 was indicated the high performance of H₂ fermentation of sugarcane bagasse hydrolysate by K. pneumoniae subsp. Pneumonia DSM 30104(T) under the optimal condition obtained.

• Periodical: Key Engineering Materials (Volume 757)
• Pages: 146-150
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.757.146
• Online since: October 2017
• Authors: Piyawadee Saraphirom*, Mullika Teerakul, Manit Anyabho
• Keywords: Box-Behnken Design, Hydrogen Production, Klebsiella pneumoniae, Plackett-Burman Design, Sugarcane Bagasse Hydrolysate

References

[1] T. Kato, M. Kubota, N. Kobayashi, Y. Suzuoki. Effective utilization of by-product oxygen from electrolysis hydrogen production. Energy. 30 (2005) 2580-2595.

DOI: 10.1016/j.energy.2004.07.004

[2] A. Fangkum, A. Reungsang. Biohydrogen production from sugarcane bagasse hydrolysate by elephant dung: Effects of initial pH and substrate concentration. Int J Hydrogen Energy. 119 (2010) 379-945.

DOI: 10.1016/j.ijhydene.2010.05.119

[3] E.K. C . Yu, N. Levitin, J.N. Saddler. Production of 2, 3-butanediol by Klebsiella pneumoniae grown on acid hydrolyzed wood hemicellulose. Biotechnology Letters. 4 (1982) 741-746.

DOI: 10.1007/bf00134670

[4] J.N. Saddler, E.C.K. Yu, M. Mes-Hartree, N. Levitin, H.H. Brownell. Utilization of enzymatically hydrolyzed wood hemicelluloses by microorganisms for production of liquid fuels. Applied and Environmental Microbiology. 45 (1983) 153-160.

DOI: 10.1128/aem.45.1.153-160.1983

[5] C.M. Pan, Y.T. Fan, Y. Xing, H.W. Hou, T. Zhang. Statistical optimization of process parameters on biohydrogen production from glucose by Clostridium sp. Fanp2. Bioresource Technol. 99 (2008) 3146-3154.

DOI: 10.1016/j.biortech.2007.05.055

[6] P. Saraphirom, A. Reungsang. Optimization of biohydrogen production from sweet sorghum syrup using statistical methods. Int J Energy. 35 (2010) 3435-3444.

DOI: 10.1016/j.ijhydene.2009.11.122

[7] Y.R. Abdel-Fattah, Z.A. Olama. L-Asparaginase production by Pseudomonas aeruginosa in solid-state culture: Evaluation and optimization of culture conditions using factorial designs. Process Biochem 38 (2002) 115-122.

DOI: 10.1016/s0032-9592(02)00067-5

[8] H. Shiratori, H. Ohiwa, H. Ikeno, S. Ayame, N. Kataoka, A. Miya, T. Beppu, K. Ueda. Lutispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming bacterium isolated from a thermophilic methanogenic bioreactor digesting municipal solid wastes. International Journal of Systematic and Evolutionary Microbiology. 58 (2008).

DOI: 10.1099/ijs.0.65490-0

[9] P. Saraphirom, A. Reungsang. Effect of organic loading rate on biohydrogen production from sweet sorghum syrup by anaerobic mixed cultures in anaerobic sequencing bath reactor. Int J Energy. 4 (2011) 55-62.

DOI: 10.1016/j.ijhydene.2010.08.058

[10] S.K. Khanal, W.H. Chen, L. Li, S. Sung. Biological hydrogen production: effects of pH and intermediate products. Int J Hydrogen Energy. (29) 2004 1123–1131.

[11] M. Ferchichi, E. Crabbe, W. Hintz, G.H. Gil, A. Almadidy. Influence of culture parameters on biological hydrogen production by Clostridium saccharoperbutylacetonicum ATTC 27021. World J Microb Biotechnol. 21 (2005) 855-862.

DOI: 10.1007/s11274-004-5972-0

[12] L.B. Kamalaskar, P.K. Dhakephalkar, K.K. Meher, D.R. Ranade. High biohydrogen yielding Clostridium sp. DMHC-10 isolated from sludge of distillery waste treatment plant. Int J Hydrogen Energy 35 (2010) 10639-10644.

DOI: 10.1016/j.ijhydene.2010.05.020

[13] E.M.R. Rises, G.G. Stewart. The effect of increased magnesium and calcium concentration on yeast fermentation performance in high gravity worts. Journal of the Institute of Brewing. 103 (1997) 287-291.

DOI: 10.1002/j.2050-0416.1997.tb00958.x