Aligned Collagen Scaffolds Enhance Tenogenic Differentiation of Bone Marrow-Derived Stem Cells

Abstract:

Tendon repair remains a formidable challenge in clinic, due to its limited function in long-term recovery. Decellularized native tendon extracellular matrix (ECM), which contains specific bioactive factors and a large number of collagen fibers, seeded with bone marrow-derived stem cells (BMSCs) has been found to promote tendon neogenesis. However, the effect of arrangements of natural collagen fibers on the differentiation of BMSCs remains unclear. Here, we prepared aligned collagen fiber scaffolds from rat tail tendon ECM to investigate the effect of collagen arrangements on the morphology, proliferation and differentiation of rat BMSCs in vitro. It was found that BMSCs were elongated and aligned along the collagen fibers on the aligned collagen fibers. Furthermore, the aligned collagen scaffolds significantly enhanced the expression of tenocyte markers TNMD and THBS4. The results indicated that aligned collagen scaffolds could induce the tenogenesis of BMSCs, which would provide an alternative approach for tendon tissue engineering.

• Periodical: Key Engineering Materials (Volume 773)
• Pages: 333-337
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.773.333
• Online since: July 2018
• Authors: Ke-Ke Zhang, Chang Liu, Yu Long Sun*
• Keywords: Aligned, Bone Marrow-Derived Stem Cells, Decellularized Tendon Extracellular Matrix Scaffolds, Differentiation

References

[1] T. R. Duquin, C. Buyea, L. J. Bisson, Which method of rotator cuff repair leads to the highest rate of structural healing? A systematic review, Am. J. Sports Med. 38 (2010) 835-841.

DOI: 10.1177/0363546509359679

[2] C. F. Liu, L. Aschbacher-Smith, N. J. Barthelery, N. Dyment, D. Butler, C. Wylie, What we should know before using tissue engineering techniques to repair injured tendons: a developmental biology perspective, Tissue Eng. Part B, Rev. 17 (2011).

DOI: 10.1089/ten.teb.2010.0662

[3] A. J. Lomas, C. N. Ryan, A. Sorushanova, N. Shologu, A. I. Sideri, V. Tsioli, G. C. Fthenakis, A. Tzora, I. Skoufos, L. R. Quinlan, G. O'Laighin, A. M. Mullen, J. L. Kelly, S. Kearns, M. Biggs, A. Pandit, D. I. Zeugolis, The past, present and future in scaffold-based tendon treatments, Adv. Drug Deliv. Rev. 84 (2015).

DOI: 10.1016/j.addr.2014.11.022

[4] G. Yang, B. B. Rothrauff, R. S. Tuan, Tendon and ligament regeneration and repair: clinical relevance and developmental paradigm, Birth defects research. Part C, Embryo today : reviews, 99 (2013) 203-222.

DOI: 10.1002/bdrc.21041

[5] C. J. Bettinger, R. Langer, J. T. Borenstein, Engineering substrate topography at the micro- and nanoscale to control cell function, Angew. Chem. 48 (2009) 5406-5415.

DOI: 10.1002/anie.200805179

[6] L. Lv, Y. Liu, P. Zhang, X. Zhang, J. Liu, T. Chen, P. Su, H. Li, Y. Zhou, The nanoscale geometry of TiO2 nanotubes influences the osteogenic differentiation of human adipose-derived stem cells by modulating H3K4 trimethylation, Biomater. 39 (2015).

DOI: 10.1016/j.biomaterials.2014.11.002

[7] J. Padmanabhan, E. R. Kinser, M. A. Stalter, C. Duncan-Lewis, J. L. Balestrini, A. J. Sawyer, J. Schroers, T. R. Kyriakides, Engineering cellular response using nanopatterned bulk metallic glass, ACS Nano, 8 (2014) 4366-4375.

DOI: 10.1021/nn501874q

[8] P. M. Crapo, T. W. Gilbert, S. F. Badylak, An overview of tissue and whole organ decellularization processes, Biomater. 32 (2011) 3233-3243.

DOI: 10.1016/j.biomaterials.2011.01.057

[9] D. O. Freytes, J. Martin, S. S. Velankar, A. S. Lee, S. F. Badylak, Preparation and rheological characterization of a gel form of the porcine urinary bladder matrix, Biomater. 29 (2008) 1630-1637.

DOI: 10.1016/j.biomaterials.2007.12.014