Effects of Extraction Conditions on Total Phenolic Content and Total Flavonoid Content of Roselle (Hibiscus sabdariffa L.) Extracts

Abstract:

The objective of this study was to determine the optimal value of factors affecting the extraction of compounds such as, polyphenols, flavonoids of Roselle. The investigated factors included extraction temperatures (30, 40, 50, 60, 70 and 80°C), extraction time (30, 60, 90, 120 and 150 minutes) and solid to solvent ratio (1:5, 1:10, 1:20, 1:40 and 1:50 g/mL). The efficiency of extraction was evaluated based on total phenolic content (TPC) and total flavonoid content (TFC). The results showed that temperature range of 60–80°C was the appropriate temperature for TFC while extraction temperature over 60°C was suitable for achieving highest TPC. Considering the effect of extraction time, duration of 90–150 minutes facilitated the extraction of both phenolics and flavonoids. Moreover, the solid to solvent 1:20 was chosen for extraction process based on the balance of antioxidant contents and their yield.

• Periodical: Key Engineering Materials (Volume 814)
• Pages: 469-474
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.814.469
• Online since: July 2019
• Authors: Nguyen Quoc Duy*, Mai Le Thanh Binh, Ma Thuan, Ngo Thi Thanh Van, Tri Duc Lam, Thien Hien Tran, Phu Nguyen Thuong Nhan
• Keywords: Extraction, Flavonoid, Hibiscus sabdaraffa L., Phenolic

References

[1] K. Raghu, Y. Naidoo, Y. H. Dewir, Secretory structures in the leaves of Hibiscus sabdariffa L., South African Journal of Botany, 121 (2019) 16-25.

DOI: 10.1016/j.sajb.2018.08.018

[2] Ghazala Riaz, Rajni Chopra, A review on phytochemistry and therapeutic uses of Hibiscus sabdariffa L., Biomedicine & Pharmacotherapy,102 (2018) 575-586.

DOI: 10.1016/j.biopha.2018.03.023

[3] Siriphan Sukkhaeng, Somnuk Promdang, Uthaiwan Doung-ngern, Fruit characters and physico-chemical properties of roselle (Hibiscus sabdariffa L.) in Thailand—A screening of 13 new genotypes, Journal of Applied Research on Medicinal and Aromatic Plants, 11(2018) 47-53.

DOI: 10.1016/j.jarmap.2018.10.001

[4] Inês Da-Costa-Rocha, Bernd Bonnlaender, Hartwig Sievers, Ivo Pischel, Michael Heinrich, Hibiscus sabdariffa L. – A phytochemical and pharmacological review, Food Chemistry, 165 (2014) 424-443.

DOI: 10.1016/j.foodchem.2014.05.002

[5] Maria Antónia Nunes, Francisca Rodrigues, Rita C. Alves, Maria Beatriz P. P. Oliveira, Herbal products containing Hibiscus sabdariffa L., Crataegus spp., and Panax spp.: Labeling and safety concerns, Food Research International 100 (2017) 529-540.

DOI: 10.1016/j.foodres.2017.07.031

[6] Emad Mohamed Abdallah, Antibacterial activity of Hibiscus sabdariffa L. calyces against hospital isolates of multidrug resistant Acinetobacter baumannii, Journal of Acute Disease, 5 2016 512-516.

DOI: 10.1016/j.joad.2016.08.024

[7] Raheem Mohssin Shadhan, Siti Pauliena Mohd Bohari, Effects of Hibiscus sabdariffa Linn. fruit extracts on α-glucosidase enzyme, glucose diffusion and wound healing activities, Asian Pacific Journal of Tropical Biomedicine, 7 (2017) 466-472.

DOI: 10.1016/j.apjtb.2017.01.023

[8] Heba A. Sindi, Lisa J. Marshall, Michael R. A. Morgan, Comparative chemical and biochemical analysis of extracts of Hibiscus sabdariffa, Food Chemistry, 164 (2014) 23-29.

DOI: 10.1016/j.foodchem.2014.04.097

[9] Pi-Jen Tsai, John McIntosh, Philip Pearce, Blake Camden, Brian R Jordan, Anthocyanin and antioxidant capacity in Roselle (Hibiscus Sabdariffa L.) extract, Food Research International, 35 (2002) 351-356.

DOI: 10.1016/s0963-9969(01)00129-6

[10] Soledad Guardiola, Núria Mach, Therapeutic potential of Hibiscus sabdariffa: A review of the scientific evidence, Endocrinología y Nutrición (English Edition), 61 274-295.

DOI: 10.1016/j.endoen.2014.04.003

[11] V. L. Singleton, R. Orthofer, and R. M. Lamuela-Raventós, Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent,, in Methods in enzymology, 299 (1999) 152–178.

DOI: 10.1016/s0076-6879(99)99017-1

[12] Y. Gong, Z. Hou, Y. Gao, Y. Xue, X. Liu, and G. Liu, Optimization of extraction parameters of bioactive components from defatted marigold (Tagetes erecta L.) residue using response surface methodology,, Food Bioprod. Process., 91 (2012) 9–16.

DOI: 10.1016/j.fbp.2010.12.004

[13] I. F. Strati and V. Oreopoulou, Effect of extraction parameters on the carotenoid recovery from tomato waste,, Int. J. food Sci. Technol., 46 (2011) 23–29.

DOI: 10.1111/j.1365-2621.2010.02496.x

[14] N. E. Durling et al., Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol–water mixtures,, Food Chem.,101(2007) 1417–1424.

DOI: 10.1016/j.foodchem.2006.03.050

[15] E. M. Silva, H. Rogez, and Y. Larondelle, Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology,, Sep. Purif. Technol., 55 (2007) 381–387.

DOI: 10.1016/j.seppur.2007.01.008

[16] Z. Y. Ju and L. R. Howard, Effects of solvent and temperature on pressurized liquid extraction of anthocyanins and total phenolics from dried red grape skin,, J. Agric. Food Chem., 51 (2003) 5207–5213.

DOI: 10.1021/jf0302106

[17] J. Wang, B. Sun, Y. Cao, Y. Tian, and X. Li, Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran,, Food Chem., 106 (2008) 804–810.

DOI: 10.1016/j.foodchem.2007.06.062

[18] M.-N. Maillard, M.-H. Soum, P. Boivin, and C. Berset, Antioxidant activity of barley and malt: relationship with phenolic content,, LWT-Food Sci. Technol., 29, (1996) 238–244.

DOI: 10.1006/fstl.1996.0035

[19] L. S. Kassama, J. Shi, and G. S. Mittal, Optimization of supercritical fluid extraction of lycopene from tomato skin with central composite rotatable design model,, Sep. Purif. Technol., 60 (2008) 278–284.

DOI: 10.1016/j.seppur.2007.09.005

[20] J. Xie, M. Xie, M. Shen, S. Nie, C. Li, and Y. Wang, Optimisation of microwave‐assisted extraction of polysaccharides from Cyclocarya paliurus (Batal.) Iljinskaja using response surface methodology,, J. Sci. Food Agric., 90 (2010) 1353–1360.

DOI: 10.1002/jsfa.3935

[21] M. Naczk and F. Shahidi, Extraction and analysis of phenolics in food,, J. Chromatogr. A, 105 (2004) 95–111.

[22] Y. Y. Thoo, S. K. Ho, J. Y. Liang, C. W. Ho, and C. P. Tan, Effects of binary solvent extraction system, extraction time and extraction temperature on phenolic antioxidants and antioxidant capacity from mengkudu (Morinda citrifolia),, Food Chem., 120 (2010) 290–295.

DOI: 10.1016/j.foodchem.2009.09.064

[23] P.-J. Tsai, J. McIntosh, P. Pearce, B. Camden, and B. R. Jordan, Anthocyanin and antioxidant capacity in Roselle (Hibiscus sabdariffa L.) extract,, Food Res. Int 35 (2002) 351–356.

DOI: 10.1016/s0963-9969(01)00129-6

[24] C. Liyana-Pathirana and F. Shahidi, Optimization of extraction of phenolic compounds from wheat using response surface methodology,, Food Chem., 93 (2005) 47–56.

DOI: 10.1016/j.foodchem.2004.08.050

[25] J. Zhou et al., Optimization of ultrasonic-assisted extraction and radical-scavenging capacity of phenols and flavonoids from Clerodendrum cyrtophyllum Turcz leaves,, PLoS One, 8 (2013).

DOI: 10.1371/journal.pone.0068392

[26] M. Vinatoru, An overview of the ultrasonically assisted extraction of bioactive principles from herbs,, Ultrason. Sonochem., 8 (2001) 303–313.

DOI: 10.1016/s1350-4177(01)00071-2

[27] D. D. Gertenbach, Solid-liquid extraction technologies for manufacturing nutraceuticals,, Funct. foods Biochem. Process. Asp., 2 (2002) 331–366.

[28] P. R. Gogate, Cavitational reactors for process intensification of chemical processing applications: A critical review,, Chem. Eng. Process.45 (2008) 515–527.

DOI: 10.1016/j.cep.2007.09.014

[29] V. M. Kulkarni and V. K. Rathod, A novel method to augment extraction of mangiferin by application of microwave on three phase partitioning,, Biotechnol. Reports, 6 (2015).

DOI: 10.1016/j.btre.2014.12.009