Synthesis and Characterization of a Polymer Matrix of Carrageenan, Chitosan and Hyaluronan on Stainless Steel 316L

Abstract:

A novel bioactive coating matrix consisting of three biopolymers – carrageenan, chitosan, and hyaluronan, was synthesized via alternate layer-by-layer (L-b-L) self-assembly of the oppositely-charged polyelectrolytes on a 316 L SS scaffold. The L-b-L deposition was carried out by substrate immersion in each polyelectrolyte solution for various length of time with 80 mins as the optimum time based on morphological non-homogeneity of coating evaluated through SEM. Co-60 γ-irradiation of the coating was done to enhance cross-linking. To test the coating matrix’s resitstance to shear stress, the coated scaffolds were subjected to agitation of 500 to 2000 rpm in phosphate buffer solution (PBS) for 24 hours. The biopolymer surface subjected to 500 rpm agitation did not exhibit noticeable surface changes and appeared similar to that of the control sample. The optimum coating was then evaluated for hemocompatibility and demonstrated low cytotoxicity with average live cell count of 77.54% after 48 hours of incubation.

• Periodical: Key Engineering Materials (Volume 821)
• Pages: 29-35
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.821.29
• Online since: September 2019
• Authors: Jhalique Jane Fojas, Rizalinda L. De Leon*
• Keywords: Hemocompatible, Layer-by-Layer Deposition, Self-Assembly Coating

References

[1] C. Wang, B. Yu, B. Knudsen, J. Harmon, F. Mouissy, Y. Mouss,. Synthesis and performance of novel hydrogels coatings for implantable glucose sensors, Biomacromolecules, 9(2) (2008) 561-567.

DOI: 10.1021/bm701102y

[2] H.H. Goreish, A.L. Lewis, S. Rose, A.W. Lloyd, The effect of phosphorylcholine-coated materials on the inflammatory response and fibrous capsule formation: in vitro and in vivo observations, J. Biomed. Mater. Res. A. 68(1) (2004) 1-9.

DOI: 10.1002/jbm.a.10141

[3] R. Michel, S. Pasche, M. Textor, D.G. Castner, Influence of PEG architecture on protein adsorption and conformation, Langmuir, 21(26) (2005) 12327-12332.

DOI: 10.1021/la051726h

[4] C.M. Nolan, C.D. Reyes, J.D. Debord, A.J. Garcia, L.A. Lyon, Phase transition behavior, protein adsorption, and cell adhesion resistance of poly(ethylene glycol) cross-linked microgel particles, Biomacromolecules, 6(4) (2005) 2032-2039.

DOI: 10.1021/bm0500087

[5] P. Kingshott, H.J. Griesser, Surfaces that resist bioachesion, Current Opinion in Solid State and Materials Science, 4(4) (1999) 403-412.

DOI: 10.1016/s1359-0286(99)00018-2

[6] J.J.R. Fojas, Development of Novel Biopolymers for Bioactive Coating of Implantable Medical Device and Drug Delivery,, PhD diss., University of the Philippines, (2017).

[7] J.J.R. Fojas, R.L. de Leon, L.V. Abad, Effects of Irradiation to Morphological, Physicochemical and Biocompatibility Properties of Carrageenan, International Journal of Biotechnology and Bioengineering, 7(5) (2013) 320-323.