The Effect of Sintering Aid on Fabrication of Three-Dimensional Carbonated Hydroxyapatite Porous Scaffolds

Abstract:

Three-dimensional (3D) carbonated hydroxyapatite (CHA) porous scaffolds were successfully fabricated via polyurethane (PU) replication technique. Two sets of porous CHA scaffolds were prepared using: 1) as-synthesized CHA slurry (SCHA) and (2) as-synthesized CHA slurry with the addition of sintering aid, magnesium hydroxide (SCHA+Mg (OH)₂). The aim of this study was to investigate the influences of the addition of sintering aid in the fabrication of porous CHA scaffolds in terms of phase purity, crystallinity, architecture, and mechanical properties. Result suggested that both of the fabricated porous scaffolds remained as single phase B-type CHA and free of secondary phases. Interestingly, the use of Mg (OH)₂ as sintering aid led to better internal architecture resulted in smoother surface and less micro-cracks/pores formation on the struts since the struts was found to be more densified as compared to SCHA scaffolds. In terms of mechanical properties, SCHA+ Mg (OH)₂ scaffolds showed higher compressive strength, indicating that the use of Mg (OH)₂ had successfully reduced the sintering temperature and improve the densification of porous scaffolds. Thus, SCHA+ Mg (OH)₂ scaffolds was found to be a better choice of scaffold with respect to its handling, compaction strength and architecture with improve strut properties.

• Periodical: Key Engineering Materials (Volume 821)
• Pages: 3-9
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.821.3
• Online since: September 2019
• Authors: Muhammad Syazwan Mohd Noor, Ahmad Fauzi Mohd Noor, Yanny Marliana Baba Ismail*
• Keywords: Bone Tissue Engineering, Carbonated, Hydroxyapatite, Porous Scaffold, Sintering Aid

References

[1] A. R. Amini, C. T. Laurencin, and S. P. Nukavarapu, Bone Tissue Engineering: Recent Advances and Challenges,, Crit. Rev. Biomed. Eng., vol. 40, no. 5, p.363–408, (2012).

DOI: 10.1615/critrevbiomedeng.v40.i5.10

[2] J. M. Bouler, P. Pilet, O. Gauthier, and E. Verron, Biphasic calcium phosphate ceramics for bone reconstruction: A review of biological response,, Acta Biomater., vol. 53, p.1–12, (2017).

DOI: 10.1016/j.actbio.2017.01.076

[3] M. S. Kamath, S. S. S. J. Ahmed, M. Dhanasekaran, and S. W. Santosh, Bone Regeneration Based on Tissue Engineering Conceptions – A 21st Century Perspective,, Int. J. Nanomedicine, vol. 9, no. 1, p.183–95, (2014).

[4] G. Thanabalasundaram, N. Arumalla, H. D. Tailor, and W. S. Khan, Regulation of differentiation of mesenchymal stem cells into musculoskeletal cells.,, Curr. Stem Cell Res. Ther., vol. 7, no. 2, p.95–102, (2012).

DOI: 10.2174/157488812799218974

[5] E. Gómez-Barrena, P. Rosset, D. Lozano, J. Stanovici, C. Ermthaller, and F. Gerbhard, Bone fracture healing: Cell therapy in delayed unions and nonunions,, Bone, vol. 70, p.93–101, (2015).

DOI: 10.1016/j.bone.2014.07.033

[6] Y. Liu, J. Lim, and S. H. Teoh, Review: Development of clinically relevant scaffolds for vascularised bone tissue engineering,, Biotechnol. Adv., vol. 31, no. 5, p.688–705, (2013).

DOI: 10.1016/j.biotechadv.2012.10.003

[7] S. Tajbakhsh and F. Hajiali, A comprehensive study on the fabrication and properties of biocomposites of poly(lactic acid)/ceramics for bone tissue engineering,, Mater. Sci. Eng. C, vol. 70, p.897–912, (2016).

DOI: 10.1016/j.msec.2016.09.008

[8] G. Tetteh, A. S. Khan, R. M. Delaine-Smith, G. C. Reilly, and I. U. Rehman, Electrospun polyurethane/hydroxyapatite bioactive Scaffolds for bone tissue engineering: The role of solvent and hydroxyapatite particles,, J. Mech. Behav. Biomed. Mater., vol. 39, p.95–110, (2014).

DOI: 10.1016/j.jmbbm.2014.06.019

[9] H. R. Ramay and M. Zhang, Preparation of porous hydroxyapatite scaffolds by combination of the gel-casting and polymer sponge methods,, Biomaterials, vol. 24, no. 19, p.3293–3302, (2003).

DOI: 10.1016/s0142-9612(03)00171-6

[10] N. Monmaturapoj and C. Yatongchai, Influence of preparation method on hydroxyapatite porous scaffolds,, Bull. Mater. Sci., vol. 34, no. 7, p.1733–1737, (2011).

DOI: 10.1007/s12034-011-0384-x

[11] Š. Monika, Substituted hydroxyapatites for biomedical applications : A review Substituted hydroxyapatites for biomedical applications : A review,, Ceram. Int., vol. 41, no. 8, p.9203–9231, (2015).

DOI: 10.1016/j.ceramint.2015.03.316

[12] L. T. Bang, B. D. Long, and R. Othman, Carbonate Hydroxyapatite and Silicon-Substituted Carbonate Hydroxyapatite : Synthesis, Mechanical Properties , and Solubility Evaluations,, vol. 2014, (2014).

DOI: 10.1155/2014/969876

[13] W. H. G. Yang, X. F. Xi, J. F. Li, and K. Y. Cai, Comparison of crystal structure between carbonated hydroxyapatite and natural bone apatite with theoretical calculation,, Asian J. Chem., vol. 25, no. 7, p.3673–3678, (2013).

DOI: 10.14233/ajchem.2013.13709

[14] W. Suchanek, M. Yashima, M. Kakihana, and M. Yoshimura, Hydroxyapatite ceramics with selected sintering additives,, Biomaterials, vol. 18, no. 13, p.923–933, (1997).

DOI: 10.1016/s0142-9612(97)00019-7

[15] I. Ezekiel, S. R. Kasim, Yanny Marliana B.I., and Ahmad-Fauzi M.N, Nanoemulsion synthesis of carbonated hydroxyapatite nanopowders: Effect of variant CO32−/PO43−molar ratios on phase, morphology, and bioactivity,, Ceram. Int., vol. 44, no. 11, p.13082–13089, (2018).

DOI: 10.1016/j.ceramint.2018.04.128

[16] Yanny Marliana B.I., I. Wimpenny, O. Bretcanu, K. Dalgarno, and A. J. El Haj, Development of multisubstituted hydroxyapatite nanopowders as biomedical materials for bone tissue engineering applications,, J. Biomed. Mater. Res. - Part A, p.1–11, (2017).

DOI: 10.1002/jbm.a.36038

[17] Yanny Marliana B.I. and Ahmad-Fauzi M.N., Effect of a novel approach of sintering on physical properties of carbonated hydroxyapatite.,, J. Mater. Sci. Eng. B, vol. 1, no. 2, p.157–163, (2011).

[18] W. Y. Wong and Ahmad-Fauzi M.N., Synthesis and Sintering-wet Carbonation of Nano-sized Carbonated Hydroxyapatite,, Procedia Chem., vol. 19, p.98–105, (2016).

DOI: 10.1016/j.proche.2016.03.121

[19] A. B. H. Yoruc, A. Karakas, E. Ayas, and A. Koyun, Effect of precipitation method on properties of hydroxyapatite powders,, Acta Phys. Pol. A, vol. 123, no. 2, p.371–373, (2013).

DOI: 10.12693/aphyspola.123.371

[20] I. Uysal, F. Severcan, A. Tezcaner, and Z. Evis, Co-doping of hydroxyapatite with zinc and fluoride improves mechanical and biological properties of hydroxyapatite,, Prog. Nat. Sci. Mater. Int., vol. 24, no. 4, p.340–349, (2014).

DOI: 10.1016/j.pnsc.2014.06.004

[21] D. Liu, J. Zhuang, C. Shuai, and S. Peng, Mechanical properties' improvement of a tricalcium phosphate scaffold with poly-L-lactic acid in selective laser sintering,, Biofabrication, vol. 5, no. 2, (2013).

DOI: 10.1088/1758-5082/5/2/025005

[22] S. H. Min, H. H. Jin, H. Y. Park, I. M. Park, H. C. Park, and S. Y. Yoon, Preparation of Porous Hydroxyapatite Scaffolds for Bone Tissue Engineering,, Mater. Sci. Forum, vol. 510–511, p.754–757, (2006).

DOI: 10.4028/www.scientific.net/msf.510-511.754