Crude Fucoidan Activity Extracted from Sargassum sp. as Mycotoxin (T-2) Binder

Abstract:

Fucoidan is a sulfated polysaccharide which is mainly found in brown seaweed like Sargassum sp. In this study, fucoidan was extracted with two distinct methods, one was using 0.1 N HCl (Fucoidan A) and the other 0.1 N H₃PO₄ (Fucoidan B). The yield of HCl extraction was respectively 7.50% and H₃PO₄ extraction 7.02%. Characterization of crude fucoidan was carried out through fourier-transform infrared spectroscopy (FTIR), followed by total carbohydrate and total sulfate level measurement. Crude fucoidan was then used to determine its binding activity against Trichothecenes T-2 toxin. Quantitative analysis of 50 ug/ml T-2 toxin binding capacity shows an efficiency of 59.52% at pH 3.0 and 58.37% at pH 6.8 for crude fucoidan A. As for crude fucoidan B, efficiency of T-2 toxin binding capacity has value of 58.12% at pH 3.0 and 57.33% at pH 6.8. Meanwhile, Commercial crude fucoidan extract from Fucus vesiculosus sp. was used as control for T-2 toxin binding capacity analysis with efficiency of 57.31% at pH 3.0 and 56.64% at pH 6.8. Therefore, fucoidan from Sargassum sp. can be utilized better as mycotoxin especially T-2 toxin binder than from Fucus vesiculosus sp. through efficiency result of fucoidan binding capacity.

• Periodical: Key Engineering Materials (Volume 840)
• Pages: 193-198
• DOI: https://doi.org/10.4028/www.scientific.net/KEM.840.193
• Online since: April 2020
• Authors: Marta Yusfita Sari, Endang Saefudin*, Fitri Fagatella
• Keywords: Fucoidan, Mycotoxine, Sargassum sp, T-2 Toxin, Trichothecenes

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