Mechanical Property Evaluation of PLA/Soybean Oil Epoxidized Acrylate Three-Dimensional Scaffold in Bone Tissue Engineering

Article Preview

Abstract:

Recently investigated photocurable, biocompatible plant resin on tissue engineering to provide the scaffold with structural support and mechanical properties. A novel method had been used here to build our scaffold by combined the traditional three-dimensional fused deposition modeling (FDM) printing and injected the structural scaffold after fabrication with plant-based resin. The materials used are polymers a synthesized one polylactic acid and soybean oil epoxidized acrylate. The addition of soybean plant-based resin improves the adhesion and proliferation of the PLA scaffold while also providing structural support to the fabricated scaffold. The purpose of the study made optimization of printing parameters and compared different printing scaffolds to select the perfect one with preferred mechanical properties. Two designs are built (cubic design and cylinder design) to make a comparison of mechanical properties between the two designs. The novel method was used through injected soybean oil resin into the PLA scaffold by avoiding any heat and temperature rise of the resin. In the traditional method, the resin is printed using an SLA printer which exposed the resin to heating before printing, this will affect the properties of the final model in our technique temperature will eliminate by direct inject the plant-based resin into the PLA scaffold and then photocuring with ultraviolet curing device for 30 min at 405nm. Finally, the results demonstrate that after injecting PLA scaffold with soybean oil resin, the mechanical properties of the scaffold improve; additionally, the results show that the cylindrical design has more promising mechanical properties than the cubic design.

You might also be interested in these eBooks

Info:

Periodical:

Pages:

17-26

Citation:

Online since:

February 2022

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2022 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

[1] F. Ghorbani, D. Li, S. Ni, Y. Zhou, B. Yu, 3D printing of acellular scaffolds for bone defect regeneration: A review, Materials Today Communications 22 (2020) 100979–100979.

DOI: 10.1016/j.mtcomm.2020.100979

Google Scholar

[2] M. Zhang, R. Lin, X. Wang, J. Xue, C. Deng, C. Feng, . . Chang, J, 3D printing of Haversian bone-mimicking scaffolds for multicellular delivery in bone regeneration, Science Advances 6 (12) (2020) 6725–6725.

DOI: 10.1126/sciadv.aaz6725

Google Scholar

[3] B. E. Grottkau, Z. Hui, Y. Yao, Y. Pang, Rapid Fabrication of Anatomically-Shaped Bone Scaffolds Using Indirect 3D Printing and Perfusion Techniques, International Journal of Molecular Sciences 21 (1) (2020) 315–315.

DOI: 10.3390/ijms21010315

Google Scholar

[4] C. Migliaresi, A. Motta, Scaffolds for tissue engineering: biological design, materials, and fabrication, Pan Stanford, Singapore, (2014).

Google Scholar

[5] D. R. Peterson, J. D. Bronzino, Biomechanics: principles and applications, CRC Press, Nueva York, (2008).

Google Scholar

[6] F. Senatov, K. Niaza, M. Zadorozhnyy, A. Maksimkin, S. Kaloshkin, Y. Estrin, Mechanical properties and shape memory effect of 3D-printed PLA-based porous scaffolds, Journal of the Mechanical Behavior of Biomedical Materials 57 (2016) 139–148.

DOI: 10.1016/j.jmbbm.2015.11.036

Google Scholar

[7] R. Trombetta, J. A. Inzana, E. M. Schwarz, S. L. Kates, H. A. Awad, 3D Printing of Calcium Phosphate Ceramics for Bone Tissue Engineering and Drug Delivery, Annals of Biomedical Engineering 45 (1) (2016) 23–44.

DOI: 10.1007/s10439-016-1678-3

Google Scholar

[8] F. Senatov, K. Niaza, A. Stepashkin, S. Kaloshkin, Low-cycle fatigue behavior of 3d- printed PLA-based porous scaffolds, Composites Part B: Engineering 97 (2016) 193–200.

DOI: 10.1016/j.compositesb.2016.04.067

Google Scholar

[9] A. Gregor, E. Filová, M. Novák, J. Kronek, H. Chlup, M. Buzgo, J. Hošek, Designing of PLA scaffolds for bone tissue replacement fabricated by ordinary commercial 3D printer, Journal of Biological Engineering (1) (2017) 11–11.

DOI: 10.1186/s13036-017-0074-3

Google Scholar

[10] G. Pezzotti, Advanced materials for joint implants, Pan Stanford Publishing, Singapore, (2013).

Google Scholar

[11] R. S. Snell, Clinical anatomy by regions, Wolters Kluwer/Lippincott Williams & Wilkins, Philadelphia, (2012).

Google Scholar

[12] N. Sultana, Biodegradable Polymer-Based Scaffolds for Bone Tissue Engineering, Springer, Berlin, Heidelberg, (2013).

Google Scholar

[13] T. Uyar, E. Kny, Electrospun Materials for Tissue Engineering and Biomedical Applications Research, Design and Commercialization, Elsevier Science, San Diego, (2017).

Google Scholar

[14] C. L. Vanputte, J. L. Regan, A. F. Russo, Seeleys essentials of anatomy and physiology, McGraw-Hill, (2010).

Google Scholar

[15] R. Enugopalan, M. Wu, Medical device materials I: proceedings from the materials and processes for medical devices conference (2005).

Google Scholar

[16] Q. Wang, Smart materials for tissue engineering: applications, Royal Society of Chemistry, London, (2017).

Google Scholar

[17] H. Xu, D. Han, J. S. Dong, G. X. Shen, G. Chai, Z. Y. Yu, Ai, Rapid prototyped PGA/PLA scaffolds in the reconstruction of mandibular condyle bone defects, The International Journal of Medical Robotics and Computer Assisted Surgery (2009).

DOI: 10.1002/rcs.290

Google Scholar

[18] E. Saito, H. Kang, J. M. Taboas, A. Diggs, C. L. Flanagan, S. J. Hollister, Experimental and computational characterization of designed and fabricated 50:50 PLGA porous scaffolds for human trabecular bone applications, Journal of Materials Science: Materials in Medicine 21 (8) (2010) 2371–2383.

DOI: 10.1007/s10856-010-4091-8

Google Scholar

[19] H. Zhou, J. G. Lawrence, S. B. Bhaduri, Fabrication aspects of PLA-CaP/PLGA-CaP com- posites for orthopedic applications: A review, Acta Biomaterialia 8 (6) (2012) 1999–(2016).

DOI: 10.1016/j.actbio.2012.01.031

Google Scholar

[20] T. Serra, J. Planell, M. Navarro, High-resolution PLA-based composite scaffolds via 3-D printing technology, Acta Biomaterialia 9 (3) (2013) 5521–5530.

DOI: 10.1016/j.actbio.2012.10.041

Google Scholar

[21] R. Kossowsky, N. Kossovsky, Advances in materials science and implant orthopedic surgery, Springer Verlag, (2013).

Google Scholar

[22] A. W. Batchelor, M. Chandrasekaran, Service characteristics of biomedical materials and implants, Imperial College Press, Singapore, (2004).

Google Scholar

[23] R. Veeramani, SJ. Holla, S. Chumber, Grays Anatomy For Students, Elsevier Health Sciences, (2017).

Google Scholar

[24] S. H. Park, D. S. Park, J. W. Shin, Y. G. Kang, H. K. Kim, T. R. Yoon, J. W. Shin, Scaffolds for bone tissue engineering fabricated from two different materials by the rapid prototyping technique: PCL versus PLGA, Journal of Materials Science: Materials in Medicine 23 (11) (2012) 2671–2678.

DOI: 10.1007/s10856-012-4738-8

Google Scholar

[25] P. Gentile, V. Chiono, I. Carmagnola, P. Hatton, An Overview of Poly(lactic-co-glycolic) Acid (PLGA)-Based Biomaterials for Bone Tissue Engineering, International Journal of Molecular Sciences 15 (3) (2014) 3640–3659.

DOI: 10.3390/ijms15033640

Google Scholar

[26] J. P. Temple, D. L. Hutton, B. P. Hung, P. Y. Huri, C. A. Cook, R. Kondragunta, W. L. Grayson, Engineering anatomically shaped vascularized bone grafts with hASCs and 3D- printed PCL scaffolds, Journal of Biomedical Materials Research Part A (2014).

DOI: 10.1002/jbm.a.35107

Google Scholar

[27] G. H. Wu, S. H. Hsu, Review: Polymeric-Based 3D Printing for Tissue Engineering, Journal of Medical and Biological Engineering 35 (3) (2015) 285–292.

DOI: 10.1007/s40846-015-0038-3

Google Scholar

[28] N. Eliaz, Degradation of implant materials, Springer, New York, (2012).

Google Scholar

[29] J. An, J. E. M. Teoh, R. Suntornnond, C. K. Chua, Design and 3D printing of scaffolds and tissues, Engineering 1 (2) (2015) 261–268.

DOI: 10.15302/j-eng-2015061

Google Scholar

[30] E. B. Bae, K. H. Park, J. H. Shim, H. Y. Chung, J. W. Choi, J. J. Lee, J. B. Huh, Efficacy of rhBMP-2 Loaded PCL/β-TCP/bdECM Scaffold Fabricated by 3D Printing Technology on Bone Regeneration, BioMed Research International (2018) 1–12.

DOI: 10.1155/2018/2876135

Google Scholar

[31] Recent Advanced in Biomaterials for 3D Printing and Tissue Engineering, Journal of Functional biomaterials 9 (2018) 22–22.

Google Scholar

[32] O. Tao, J. Kort-Mascort, Y. Lin, H. M. Pham, A. M. Charbonneau, O. A. Elkashty, S. D. Tran, The Applications of 3D Printing for Craniofacial Tissue Engineering, Micromachines 10 (7) (2019) 480–480.

DOI: 10.3390/mi10070480

Google Scholar

[33] L. Chen, L. Shao, F. Wang, Y. Huang, F. Gao, Enhancement in sustained release of an- timicrobial peptide and BMP-2 from degradable three dimensional-printed PLGA scaffold for bone regeneration, RSC Advances 9 (19) (2019).

DOI: 10.1039/c8ra08788a

Google Scholar

[34] B. Leukers, H. Gülkan, S. H. Irsen, S. Milz, C. Tille, M. Schieker, H. Seitz, Hydroxyapatite scaffolds for bone tissue engineering made by 3D printing, Journal of Materials Science: Materials in Medicine 16 (12)(2005) 1121–1124.

DOI: 10.1007/s10856-005-4716-5

Google Scholar

[35] F. C. Fierz, F. Beckmann, M. Huser, S. H. Irsen, B. Leukers, F. Witte, . . Müller, B, The morphology of anisotropic 3D-printed hydroxyapatite scaffolds, Biomaterials 29 (28) (2008) 3799–3806.

DOI: 10.1016/j.biomaterials.2008.06.012

Google Scholar

[36] S. Bose, S. Vahabzadeh, A. Bandyopadhyay, Bone tissue engineering using 3D printing, Materials today 16 (12) (2013) 496–504.

DOI: 10.1016/j.mattod.2013.11.017

Google Scholar

[37] S. C. Cox, J. A. Thornby, G. J. Gibbons, M. A. Williams, K. K. Mallick, 3D printing of porous hydroxyapatite scaffolds intended for use in bone tissue engineering applications, Materials Science and Engineering: C 47 (2015) 237–247.

DOI: 10.1016/j.msec.2014.11.024

Google Scholar

[38] H. Y. He, J. Y. Zhang, X. Mi, Y. Hu, X. Y. Gu, Rapid prototyping for tissue-engineered bone scaffold by 3D printing and biocompatibility study, International journal of clinical and experimental medicine 8 (7) (2015) 11777–11777.

Google Scholar

[39] C. Wang, W. Huang, Y. Zhou, L. He, Z. He, Z. Chen, . . Wei, Y, 3D printing of bone tissue engineering scaffolds, Bioactive Materials 5 (1) (2020) 82–91.

DOI: 10.1016/j.bioactmat.2020.01.004

Google Scholar

[40] B. Bisht, A. Hope, A. Mukherjee, M. Paul, Advances in the Fabrication of Scaffold and 3D Printing of Biomimetic Bone Graft, Annals of Biomedical Engineering 49 (4) (2021) 1128–1150.

DOI: 10.1007/s10439-021-02752-9

Google Scholar