Antioxidant Activity, Antimicrobial Activities, and Effect on the Viability of Fibroblast Cells of Boesenbergia rotunda- Loaded Hydrogel Patches

Abstract:

Boesenbergia rotunda (B. rotunda) extract-loaded hydrogel patches were fabricated to be an antibacterial dressing. The biological properties such as antioxidant properties, bacterial protection activity, and the effect on normal human fibroblast (NHF) viability were assessed compared with blank hydrogel patches. The antioxidant activity of B. rotunda extract-loaded hydrogel patches was 75.00 ± 0.79 % (at 4-h treatment time), whereas the blank hydrogel patch had low antioxidant activity. The extract-loaded patches exhibited antioxidant activity approximately 16.5 times higher than that of the blank patches. In addition, the qualitative antibacterial activity of the patches was studied by agar disc diffusion. The inhibition zone of the extract-loaded hydrogel discs against Staphylococcus aureus (S. aureus) was 7.33 ± 0.58 mm, whereas the inhibition zone of the discs against Escherichia coli (E. coli) could not be observed. Moreover, the quantitative antibacterial activity showed an inhibition effect of the extract-loaded hydrogels against both types of bacteria. After incubation for 24 h, the extract-loaded hydrogel patches inhibited the growth of S. aureus and E. coli at the percentage of 49.76 ± 3.82 and 20.13 ± 2.75%, respectively. The results from the infiltration test showed that the extract-loaded hydrogel patches could prevent bacteria contamination. In addition, no toxicity to NHF cells had been observed. Therefore, the B. rotunda extract-loaded hydrogel patches could be a candidate material as an antibacterial wound dressing.

• Periodical: Key Engineering Materials (Volume 914)
• Pages: 37-43
• DOI: https://doi.org/10.4028/p-f3jgw4
• Online since: March 2022
• Authors: Pattaranut Eakwaropas*, Nandar Maung Maung Aye, Tanasait Ngawhirunpat, Boonnada Pamornpathomkul
• Keywords: Antimicrobial, Antioxidant, Boesenbergia rotunda, Fibroblast Viability, Hydrogel Patches

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