Synthesis and Characterization of Mesoporous β-Tricalcium Phosphate

Abstract:

Reconstruction of bone defect due to a disease or a trauma can use autograft, allograft, xenograft or synthetic bonegraft as the bone substitute material. However, in particular cases, it is required a material that has a specific resorption characteristic, beside owning excellent bioactive properties, such as β-tricalcium phosphate (β-TCP). In this study, we report the synthesis of β-TCP particles with mesopores structure by using chitosan and aloe vera as templates. A solution of (NH₄)₂HPO₄ was added dropwise into solution of Ca(NO₃)₂·4H₂O and the template at 85°C for 2 hours, and subsequently aged for 3 hours. Then, the formed precipitate was washed and centrifuged repeatedly prior to drying at 80°C for 24 hours. Finally, the dried precipitate was calcined at 900°C for 1 hour to obtain β-TCP powder. Phase identification and mesopores structure were analyzed using X-ray diffraction (XRD), while the existence of functional groups was identified by Fourier-transform infrared (FTIR) spectroscopy. Microstructure and particle size distribution were characterized by scanning electron microscopy (SEM) and particle size analyzer (PSA), respectively. XRD analysis shows that β-TCP is dominant with the presence of small amount of impurities. Furthermore, low angle peak in XRD analysis indicates the formation of mesopores structure. From the SEM and PSA analysis, the morphology of both TCP-K and TCP-KA particles showed more large agglomerates and more heterogeneous particle size distribution due to the addition of the biopolymers in the synthesis of β-TCP.

• Periodical: Materials Science Forum (Volume 1028)
• Pages: 359-364
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.1028.359
• Online since: April 2021
• Authors: Nendar Herdianto*, Dwi Gustiono, Riesma Tasomara, Adita Wardani Rahmania, Ika Maria Ulfa, Annisa Ramadhanti Ririanza Putri, Winda Rianti
• Keywords: Aloe Vera, Characterization, Chitosan, Mesoporous, Synthesis, β-Tricalcium Phosphate

References

[1] Horch H.H., R. Sader, C. Pautke, A. Neff, H. Deppe, A. Kolk, Synthetic, pure-phase beta-tricalcium phosphate ceramic granules (Cerasorb) for bone regeneration in the reconstructive surgery of the jaws, Int J Oral Maxillofac Surg. 2006 Aug;35(8):708-13. doi: 10.1016/j.ijom.2006.03.017. Epub 2006 May 9.

DOI: 10.1016/j.ijom.2006.03.017

[2] Horowitz, R. A., Z. Mazor, C. Foitzik, H. Prasad, M. Rohrer, A. Palti, β-tricalcium phosphate as bone substitute material: properties and clinical applications, J of Osseointegration 2010; 1(2):61-68.

[3] Kamitakahara M., C. Ohtsuki, T. Miyazaki, Review paper: behavior of ceramic biomaterials derived from tricalcium phosphate in physiological condition, J Biomater Appl. 2008 Nov;23(3):197-212. doi: 10.1177/0885328208096798.

DOI: 10.1177/0885328208096798

[4] Ducheyne P, S. Radin, L. King, The effect of calcium phosphate ceramic composition and structure on in vitro behavior. I. Dissolution, J Biomed Mater Res. 1993 Jan;27(1):25-34. doi: 10.1002/jbm.820270105.

DOI: 10.1002/jbm.820270105

[5] Bone regeneration: molecular and cellular interactions with calcium phosphate ceramics, Int. J Nanomedicine 2006:1(3) 317–332.

[6] Gu L., X. He, Z. Wu, Mesoporous hydroxyapatite: Preparation, drug adsorption, and release properties, Materials Chemistry and Physics 148 (2014):153-158.

DOI: 10.1016/j.matchemphys.2014.07.024

[7] Vallet-Regi, M., A. Ramila, R. P. del Real, and J. Pe´rez-Pariente, A New Property of MCM-4: Drug Delivery System, Chem. Mater. 2001, 13, 308-311.

DOI: 10.1021/cm0011559

[8] Mohammad, N. F., R. Othman, and Y. F. Yee, Pore characteristics of mesoporous carbonated hydroxyapatite synthesised with different nonionic surfactant and carbonate concentration, Materials Science Forum Vol 819 (2015): 353-360,.

DOI: 10.4028/www.scientific.net/msf.819.353

[9] Xidaki D., P. Agrafioti, D. Diomatari, A. Kaminari, E. Tsalavoutas-Psarras, P. Alexiou, V. Psycharis, E. C. Tsilibary, S. Silvestros, and M. Sagnou, Synthesis of Hydroxyapatite, β-Tricalcium Phosphate and Biphasic Calcium Phosphate Particles to Act as Local Delivery Carriers of Curcumin: Loading, Release and In Vitro Studies, Materials 2018, 11, 595;.

DOI: 10.3390/ma11040595

[10] Destainville, A., E. Champion, D. Bernache-Assollant, E. Laborde, Synthesis, characterization and thermal behavior of apatitic tricalcium phosphate, Materials Chemistry and Physics 80 (2003) 269–277.

DOI: 10.1016/s0254-0584(02)00466-2

[11] Singh, S., P. Bhardwaj, V. Singh, S. Aggarwal, U.K. Mandal, Synthesis of nanocrystalline calcium phosphate in microemulsion-effect of nature of surfactants, J Coll. and Int. Sci. 319 (2008):322–329.

DOI: 10.1016/j.jcis.2007.09.059

[12] Ataol, S., A. Tezcaner, O. Duygulu, D. Keskin, N. E. Machin, Synthesis and characterization of nanosized calcium phosphates by flame spray pyrolysis, and their effect on osteogenic differentiation of stem cells, J Nanopart Res (2015) 17:95, DOI 10.1007/s11051-015-2901-0.

DOI: 10.1007/s11051-015-2901-0

[13] Morsi, R.E., and R. S. Mohamed, Nanostructured mesoporous silica: influence of the preparation conditions on the physical-surface properties for efficient organic dye uptake, R. Soc. open sci. 5: 172021. http://dx.doi.org/10.1098/rsos.172021.

DOI: 10.1098/rsos.172021

[14] Ibrahim A.R., W. Wei, D. Zhang, H. Wang, J. Li, Conversion of waste egg shells to mesoporous hydroxyapatite nanoparticles with high surface area, Materials Letters110(2013)195–197.

DOI: 10.1016/j.matlet.2013.08.014