One of the important factors resulting in the high-speed machinery performance is its machine tool dynamic characteristic. It will directly influence the final manufacturing capability of the machinery and it is also the important criterion of evaluating machinery performances. Especially, the dynamic characteristic of spindle assembly has large effect on manufacturing capability of machinery. So it is very important both analyzing dynamic-static characteristic of the spindle assembly and machine tool of high-speed machinery and studying the effect to machinery function. Using Finite Element Analysis software did a Finite Element Analysis of spindle assembly of the high-speed machine tool, and some experiments are done to validate the result the Finite Element Analysis results.