The hot deformation behavior and microstructure of rolled Inconel 625 alloy have been studied from 930°C to 1180°C, and at strain rate from 10 s-1 to 80 s-1, respectively. The results indicate that, as deformation temperature rises, both peak flow stress (PFS) and recrystallization critical strain (RCS) decrease; as the strain rate increases, the PFS is enhanced, but the RCS drops. When the deformation temperature is within 1100°C and 1180°C, the grain size coarsens markedly with the temperature increasing. When the deformation temperature is lower than 1100°C，a higher strain rate is helpful for grain refinement. However, when the temperature is beyond 1100°C,the effect of strain rate on the grain size is reduced.