Measuring the Heparin Binding Capability of Polyurethane-Coated Stainless Steel Stents with a Label-Free Biosensor
In the development of modern drug eluting implants it is crucial to be able to measure the long term desorption processes which determine the drug elusion. In this article we set up a simple model for these measurements, which consists of stainless steel stent-model samples with polyurethane coatings incubated in heparin, and a label-free OWLS (Optical Waveguide Lightmode Spectroscopy) biosensor to measure the elusion of heparin from these samples. We found that porous coatings bind heparin better than smooth coatings, and that the PUR materials tested by us all have different binding properties. We also constructed a dual-flow set-up for the instrument, which enabled us to do real-time elusion measurements instead of sample injection, which would make the monitoring of desorption processes much clearer, although this measurement method is yet to be fully developed.
P. J. Szabó and T. Berecz
Z. Puskás et al., "Measuring the Heparin Binding Capability of Polyurethane-Coated Stainless Steel Stents with a Label-Free Biosensor", Materials Science Forum, Vol. 659, pp. 331-335, 2010