Osteoblast Behavior In Vitro in Porous Calcium Phosphate Composite Scaffolds, Surface Activated with a Cell Adhesive Plasma Polymer Layer

Barbara Nebe; Matthias Cornelsen; Antje Quade; Volker Weissmann; Friederike Kunz; Stefan Ofe; Karsten Schroeder; Birgit Finke; Hermann Seitz; Claudia Bergemann

doi:10.4028/www.scientific.net/MSF.706-709.566

Paper Titles

Control of Osteoblastic Cell Behavior by Surface Topography Introduced by Plastic Deformation of Ti Single Crystal with h.c.p. Structure
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Mechanical Properties of Ti-Cr-Sn-Zr Alloys with Low Young’s Modulus
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Young’s Modulus Changeable Titanium Alloys for Orthopaedic Applications
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Single Crystal Growth and its Microstructure in Co-Cr-Mo Alloys for Biomedical Applications
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Osteoblast Behavior In Vitro in Porous Calcium Phosphate Composite Scaffolds, Surface Activated with a Cell Adhesive Plasma Polymer Layer
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Collagen-Silk Fibroin Fibers: A Promising Scaffold for Vascular Tissue Engineering
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High Performance Beta Titanium Alloys as a New Material Perspective for Cardiovascular Applications
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Reconstituted Extracellular Matrix Improve Osteoblastic Differentiation onto Titanium Surfaces
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Synthesis and Characterization of Hydroxyapatite-Based Nanostructures: Nanoparticles, Nanoplates, Nanofibers and Nanoribbons
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Osteoblast Behavior In Vitro in Porous Calcium Phosphate Composite Scaffolds, Surface Activated with a Cell Adhesive Plasma Polymer Layer

Abstract:

Synthetic materials such as bone substitutes are permanently under development for applications in orthopedic and trauma surgery. Our porous scaffolds were produced from ß-tricalcium phosphate (TCP) using the three dimensional (3D)-printing technology. After sintering the porosity and the pore size of the 3D printed scaffolds reached nearly 50 % and 500 µm, respectively. TCP scaffolds were additionally stabilized by infiltration with polylactic acid (PLA). Because PLA usually impeded cell adhesion we activated the composite surface with plasma polymerized allylamine in a low temperature plasma process. For cell investigations inside the scaffold we used a module system, where two porous discs can be horizontally fixed within a clamping ring. Thereby a 3D cell culture module with four levels and a maximal height of 10 mm was generated. Human MG-63 osteoblasts (ATCC) were seeded apically and placed in serum-containing DMEM. After 14 days of a static cell culture the cell ingrowth and mobility was analyzed by scanning electron microscopy. Osteoblast's initial adhesion and short time occupation of the surface is significantly improved on plasma polymer activated TCP surfaces, which could be a precondition for an enhanced colonization inside a calcium phosphate scaffold. Interestingly, the plasma functionalization of the pure TCP scaffold was possible and successful concerning cell acceptance.