Thiolated Gd(III) Chelate Coated Gold Nanoparticles: Synthesis, Characterization, X-Ray CT and MRI Relaxivity Studies

Abstract:

Gadolinium complex of 2-aminothiophenol conjugated DTPA (DTPA=diethylenetriamine N,N,N',N",N" pentacetic acid) bis (amide) has been synthesized and characterized by various analytical techniques such as elemental analysis (EA), NMR, FAB-MS, IR, UV etc. This thiolated GdL (where L is a conjugate of DTPA and 2-aminothiophenol) has been anchored on the gold nanoparticles surfaces through thiols functionalites. These gold nanoparticles (AuNPs) have been synthesized by the reduction of gold tetrachloride (HAuCl₄) using sodium citrate as reducing agent. The surface functionalization has been performed by the replacement of citrate coat on the gold nanoparticle surface with thiolated Gd-chelate, Au@GdL. The Au@GdL has been analyzed by XRD, transmission electron microscope (HRTEM), UV, ICP-MS etc. The average size of nanoparticles is about 22 nm with a uniform spherical shape. A very high number of GdL has been loaded on nanoparticle surface reaching up to 7.9x10³ of Gd (III)- chelates per nanoparticle and they demonstrate very high r₁ relaxivity and the r₁ relaxivity per [G is much higher than the Gd (III)-chelate alone. The bimodality has also been tested using in-vitro x-ray computed tomography (x-ray CT). These nanoparticles (GNPs) are very stable and homogeneously dispersed in aqueous solution.

• Periodical: Materials Science Forum (Volume 754)
• Pages: 121-130
• DOI: https://doi.org/10.4028/www.scientific.net/MSF.754.121
• Online since: April 2013
• Authors: M. Nasiruzzaman Shaikh
• Keywords: Bimodal CAs, DTPA, Gd-Chelate, Gold Nanoparticles (GNP), MRI CAs, Polyaminocarboxylates, Thiols

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