Protein Nanoparticles with Enzymatic and Antigen-Binding Activities Induce Th1 Cytokine Gene Expression

Olga V. Morozova; Elena I. Isaeva; Dmitry V. Klinov

doi:10.4028/www.scientific.net/MSF.995.109

Paper Titles

Supercapacitors with Extended Operating Potential Window
p.84

Surface Morphology Studies on Laser Irradiated Target and Bi(Pb)SrCaCuO Thin Films
p.89

Efficiency Improvement of Gelatin-Based Wound Dressings by Silk Fibroin and Chitosan
p.97

Effect of Polylactic Acid/Hydroxyapatite Coating on Dental Implant Using Finite Element Method
p.103

Protein Nanoparticles with Enzymatic and Antigen-Binding Activities Induce Th1 Cytokine Gene Expression
p.109

Investigation on Effect of Varying Weight Percentage of Glass Fiber and Filler on the Properties of Glass Fiber-Cement- Polyester Composites
p.117

Numerical Investigation on the CFRP Strengthened Steel Frame under Earthquake
p.123

Analysis of the Effect of Fly Ash on Compressive Strength of Concrete Using Neural Network and Parameter Analysis
p.130

Experimental Study on Concrete-Filled Double Skin Circular Steel Tubular Beams under Bending Load
p.136

HomeMaterials Science ForumMaterials Science Forum Vol. 995Protein Nanoparticles with Enzymatic and...

Protein Nanoparticles with Enzymatic and Antigen-Binding Activities Induce Th1 Cytokine Gene Expression

Abstract:

To improve protein stability and membrane permeability the protein nanoparticles (NP) were fabricated from enzymes or virus-specific antibodies by nanoprecipitation. Lysozyme NP destroyed bacterial cellular walls. RNase-based NP hydrolyzed RNA. Lipase, catalase and horseradish peroxidase activities were also registered for corresponding protein NP. NP from polyclonal antibodies against the influenza A virus and monoclonal antibodies against hemagglutinin H1, H3; proteins NS and NP could interact with their specific antigens. NP from monoclonal antibodies against the hepatitis B surface antigen bound with the antigen as shown by ELISA. The protein NP were stable in water at 4°C for several months and in the presence of blood sera or saliva for more than 5 days. The protein NP were not toxic for human cells and mice. The protein NP could penetrate and accumulate in cells in 2 hours with maximum after 2 days and subsequent gradual decline until background values. Entry of foreign protein NP into the eukaryotic cells induced cytokine gene expression. RNA of interferon (IFN) a, b and l but not IFN g were detected by reverse transcription with real time PCR. Polarization index (Th2:Th1 cytokine RNA) was near 0. Accordingly, cellular uptake of non-toxic protein NP induced Th1 polarization of immune response.

You might also be interested in these eBooks

View Preview

Info:

Periodical:

Materials Science Forum (Volume 995)

Pages:

109-113

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.995.109

Citation:

Cite this paper

Online since:

June 2020

Authors:

Olga V. Morozova*, Elena I. Isaeva, Dmitry V. Klinov

Keywords:

Composite Nanoparticles, Cytokine Gene Expression, Enzyme Activity, Immune Response Polarization Indexes, Protein Nanoparticles, Tissue Cultures

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] D. Bobo, K.J. Robinson, J. Islam, K.J. Thurecht, S.R. Corrie: Pharm Res. Vol. 33(10) (2016), p.2373.

Google Scholar

[2] W. Lohcharoenkal, L. Wang, Y.C. Chen, Y. Rojanasakul: Biomed Res Int. (2014), p.180549.

Google Scholar

[3] O.V. Morozova, E.R. Pavlova, D.V. Bagrov, N.A. Barinov, K.A. Prusakov, E.I. Isaeva, V.V. Podgorsky, D.V. Basmanov, D.V. Klinov: International Journal of Nanomedicine Vol. 13 (2018), p.6637.

DOI: 10.2147/ijn.s177627

Google Scholar

[4] O.V. Morozova, T.P. Ospelnikova. Patent 2016131205, 28.07.2016. Ru 2 627 179. C12Q 1/68 (2006.01).

Google Scholar

[5] A. H. Ummu, Z. H. Mohd, B.A. Noorjahan, A. Ya. A.Syazaira, J.M. Mas: International Journal of Nanomedicine Vol. 13 (2018), p.5075.

Google Scholar