Paper Titles
Editorial
Electrokinetic and Impedimetric Dynamics of FeCo-Nanoparticles on Glassy Carbon Electrode
p.1
Study on Use of Nanosized Alpha Alumina from Schedule Waste as Formulation in Thermal Insulation Paint
p.25
Study on Bactericidal Effect of Biosynthesized Silver Nanoparticles in Combination with Gentamicin and Ampicillin on Pseudomonas aeruginosa
p.37
Light Extraction from GaN-Microcavity
p.51
Ferroelectric Properties of Strontium Bismuth Titanate (SrBi4Ti4O15) Synthesized Using Solution Combustion Technique
p.67
Thermal and Photoluminescence Properties of Nd3+ Doped Tellurite Nanoglass
p.81
Fabrication of p-Type Double Gate and Single Gate Junctionless Silicon Nanowire Transistor by Atomic Force Microscopy Nanolithography
p.93
ZnO Nanoparticles to Nanowires and Nanobundles
p.115

Study on Bactericidal Effect of Biosynthesized Silver Nanoparticles in Combination with Gentamicin and Ampicillin on Pseudomonas aeruginosa

Article Preview

Abstract:

Silver nanoparticles are the most promising nanomaterial with antibacterial properties. Recent study of resistance to most potential antibiotics promotes research in the bactericidal activity of the silver nanoparticles. In this work, the effect of biosynthesized silver nanoparticles, in combination with gentamicin and ampicillin, on Pseudomonas Aeruginosa bacteria has been studied. Pseudomonas Aeruginosa is a common bacterium that can cause infections which are generalized as inflammation and sepsis. The results show that the bactericidal properties of the nanoparticles depends on the size of the as-synthesized silver nanoparticles as nanoparticles of diameter ~120 nm only have a direct interaction with the bacteria. It is observed that the antibacterial activities of antibiotics increase in the presence of AgNPs against test strains. Silver nanoparticles were synthesized elctrolytically using silver wire of 99% purity as anode and carbon rod wrapped with LDPE as cathode. 0.01 N Silver nitrate was used as an electrolyte. The process is termed as biosynthesis, because tea extract was used used as the capping agent which is also a very mild reducing agent. The polyphenols theaflavins and thearubigins, present in tea perform the role of stabilizing or capping agents due to their bulky and steric nature. A brown colored colloidal solution of silver nanoparticles is obtained. The as-synthesized silver nanoparticles were characterized using XRD, TEM and UV-Vis spectroscopy.

By email View Pdf
You have full access to the following eBook
Nano Hybrids Vol. 3 Read eBook

Info:

Periodical:

Nano Hybrids (Volume 3)

Pages:

37-49

DOI:

https://doi.org/10.4028/www.scientific.net/NH.3.37

Citation:

Cite this paper

Online since:

January 2013

Authors:

Shweta Rajawat, M.S. Qureshi

Keywords:

Gentamicin, Pseudomonas Aeruginosa, Silver Nanoparticle, Tea Extract

Export:

RIS, BibTeX

Permissions:

Creative Commons CC BY 4.0

Share:

Citation:

References

[1] N. Saifuddin, C.W. Wong, A.A. Nur Yasumira, Rapid Biosynthesis of Silver Nanoparticles Using Culture Supernatant of Bacteria with Microwave irradiation, E-Journal of Chemistry 6(1) (2009) 61-70.

DOI: 10.1155/2009/734264

Google Scholar

[2] M. Singh, S. Singh, S. Prasada, I.S. Gambhir, Nanotechnology in medicine and antibacterial effect of silver nanoparticles, Dig. J. Nanomater. Biostruct., 3(3) (2008) 115-122.

Google Scholar

[3] A. Pal, S. Shah, S. Devi, Preparation of silver, gold and silver-gold bimetallic nanoparticles in w/o microemulsion containing TritonX-100, Colloids Surf. A 302 (2007) 483-487.

DOI: 10.1016/j.colsurfa.2007.03.032

Google Scholar

[4] M.J. Rosemary, T. Pradeep, Solvothermal synthesis of silver nanoparticles from Thiolates, J. Colloid Interface Sci. 268 (1) (2003) 81-84.

DOI: 10.1016/j.jcis.2003.08.009

Google Scholar

[5] Y. Xie, R. Ye, H. Liu, Synthesis of silver nanoparticles in reverse micelles stabilized by natural biosurfactant, Colloids Surf. A 279 (2006) 175-178.

DOI: 10.1016/j.colsurfa.2005.12.056

Google Scholar

[6] B. Soroushian, I. Lampre, J. Belloni, M. Mostafavi, Radiolysis of silver ion solutions in ethylene glycol: solvated electron and radical scavenging yields, Radiat. Phys. Chem. 72 (2005) 111-118.

DOI: 10.1016/j.radphyschem.2004.02.009

Google Scholar

[7] J.J. Zhu, X.H. Liao, X.N. Zhao, H.Y. Hen, Preparation of silver nanorods by electrochemical methods, Mater. Lett. 49 (2001) 91-95.

Google Scholar

[8] S. Liu, S. Chen, S. Avivi, A. Gedanken, Synthesis of amorphous silver nanoparticles by pulse sonoelectrochemical method, J. Non-Cryst. Solids 283 (1-3) (2001) 231-236.

DOI: 10.1016/s0022-3093(01)00362-3

Google Scholar

[9] S. Pal, Y. Kyung, S. Myong, Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli, J. Appl. Environ. Microbiol., 73(6) (2007) 1712-1720.

DOI: 10.1128/aem.02218-06

Google Scholar

[10] A. Panacek, L. Kvitek, R. Prucek, M. Kolar, R. Vecerova, N. Pizurova, V.K. Sharma, T. Nevecna, R. Zboril R, Initial Study on the Toxicity of Silver Nanoparticles (NPs) against Paramecium caudatum, J. Phys. Chem. B 110 (2006) 16248-16253.

DOI: 10.1021/jp808645e

Google Scholar

[11] M.V. Roldán, A.L. Frattini, O.A. Sanctis, N.S. Pellegrini, Metal Nanoparticles with different shapes, Anales AFA 17 (2005), 212-217.

Google Scholar

[12] Z. Zhu, L. Kai, Y. Wang, Synthesis and application of hyper branched polyesters- preparation and characterization of crystalline silver nanoparticles, Mater. Chem. Phys. 96 (2006) 447-453.

DOI: 10.1016/j.matchemphys.2005.07.067

Google Scholar

[13] A.S. Edelstein, R.C. Cammarata (Eds. ), Nanomaterials, synthesis, Properties and applications (1996), Bristol and Philadelphia Publishers, Bristol.

Google Scholar

[14] N. Duran, P.D. Marcato, O.L. Alves, G.I. Souza, E. Esposito, Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains, Journal of Nanobiotechnology 3 (2005) 8-14.

DOI: 10.1186/1477-3155-3-8

Google Scholar

[15] P.T. Anstas, J. Warner, Green Chemistry: Theory and Practice, Oxford University Press, New York, NY, USA, (1998).

Google Scholar

[16] P. Mukherjee, A. Ahmad, D. Mandal, S. Senapati, S.R. Sainkar, M.I. Khan, R. Parischa, P.V. Ajayakumar, M. Alam, R. Kumar, M. Sastry, Fungus –mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis. Nano Lett. 1 (2001).

DOI: 10.1021/nl0155274

Google Scholar

[17] M. Sastry, A. Ahmad, N.I. Islam, R. Kumar, Biosynthesis of metal nanoparticles using fungi and actinomycete, Current Sci. 85 (2003) 162-170.

Google Scholar

[18] A. Ahmad, P. Mukherjee, S. Senapati, D. Mandal, M.I. Khan, R. Kumar, M. Sastry, Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium Oxysporum, Colloids Surf. B: Biointerfaces 28 (2003) 313-318.

DOI: 10.1016/s0927-7765(02)00174-1

Google Scholar

[19] M. Sathishkumar, K. Sneha, S.W. Won, C.W. Cho, S. Kim, Y.S. Yun, Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity, Colloids Surf. B: Biointerfaces 73 (2009).

DOI: 10.1016/j.colsurfb.2009.06.005

Google Scholar

[20] A. Tripathi, N. Chandrasekaran, A.M. Raichur, A. Mukherjee, Antibacterial applications of silver nanoparticles synthesized by aqueous extract of Azadirachta indica (Neem) leaves. J. Biomed. Nanotechnol. 5 (2009) 93-98.

DOI: 10.1166/jbn.2009.038

Google Scholar

[21] T.C. Prathna, N. Chandrasekaran, A.M. Raichur, A. Mukherjee, Biomimetic synthesis of silver nanoparticles by Citrus limon (lemon) aqueous extract and theoretical prediction of particle size. Colloids Surf. B: Biointerfaces 82 (2011) 152-159.

DOI: 10.1016/j.colsurfb.2010.08.036

Google Scholar

[22] S.K. Sivaraman, I. Elango, S. Kumar, V. Santhanam, A green protocol for room temperature synthesis of silver nanoparticles in seconds, Curr. Sci. 97 (2009) 1055-1059.

Google Scholar

[23] J.Y. Song, B.S. Kim, Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst. Eng. 32 (2009) 79-84.

DOI: 10.1007/s00449-008-0224-6

Google Scholar

[24] V. K. Sharma, R.A. Yngard, Y. Lin, Silver nanoparticles: Green synthesis and their antimicrobial activities, Adv. Coll. Interf. Sci. 145 (2009) 83-96.

Google Scholar

[25] M. Bubey, S. Bhaduria, B.S. Kushwaha, Green Synthesis of Nanosilver Particles from Extract of Eucalyptus Hybrida (Safeda) Leaf, Dig. J. Nanomater. Biostruct. 4(3) (2009) 537-543.

Google Scholar

[26] E. Parameswari, C. Udayasoorian, S.P. Sebastian, R.M. Jayabalakrishnan, The bactericidal potential of silver nanoparticles, International Research Journal of Biotechnology 1(3) (2010) 44-49.

Google Scholar