The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles

Moo Eon Park; Ki Ho Kang; Kyung Ja Kim; Jeong Ho Chang

doi:10.4028/www.scientific.net/SSP.124-126.903

Paper Titles

Factors Influencing the Sensitivity of the CDMPI Sensor
p.887

Magnetic Properties of Mn-Zn Ferrite Nanoparticles Fabricated by Conventional Ball-Milling
p.891

Functionalization of Magnetite Nanoparticles for Protein Immobilization
p.895

Synthesis of Monodisperse FePt Nanoparticles at a Low Temperature
p.899

The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles
p.903

Structure and Magnetic Properties of Nanocrystalline Pd-Co and Pd-Co-Fe Layers
p.907

Edge Shape Effect on Switching Behavior in a Small Ferromagnetic Pattern
p.911

Ti-Based Amorphous Alloys and Composites as Potential Candidate Materials for Energy Applications
p.915

DLC Film Coating on a Lithium Metal as an Anode of Lithium Secondary Batteries
p.919

HomeSolid State PhenomenaSolid State Phenomena Vols. 124-126The Selective Protein Separations with...

The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles

Abstract:

This work reported the development of the high throughput protein separation process with molecularly assembled silica-coated magnetic nanoparticles as a function of amino group numbers such as mono-, di-, and tri-aminofunctionality, in which the range of silica coating thicknesses were optimized to be interacted with protein. The protein separation efficiency was demonstrated as a function of each aminofunctional group and the particle sizes of the silica coated magnetic nanoparticles. The particles were prepared by the chemical precipitation of Fe2+ and Fe3+ salts with a molar ratio of 1:2 under basic solution. The silica coated magnetic nanoparticles were directly produced by the sol-gel reaction of a tetraethyl orthosilicate (TEOS) precursor, in which the coating layer serves as a biocompatible and versatile group for further biomolecular functionalization. To effectively capture the proteins, silica coated magnetic nanoparticles need to be functionalized reproducibly on the silica surface, and three kinds of amino functional groups were adapted as a function of number of amine using the mono-, di-, and tri-aminopropylalkoxysilanes.

You might also be interested in these eBooks

Advances in Nanomaterials and Processing

View Preview

Info:

Periodical:

Solid State Phenomena (Volumes 124-126)

Pages:

903-906

DOI:

https://doi.org/10.4028/www.scientific.net/SSP.124-126.903

Citation:

Cite this paper

Online since:

June 2007

Authors:

Moo Eon Park, Ki Ho Kang, Kyung Ja Kim, Jeong Ho Chang

Keywords:

BSA, Magnetite, Protein Separation, Silica Shell

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] I. Safarik and M. Safarikova, J. Chromatogr. B Vol. 722 (1999), p.33.

Google Scholar

[2] L. Babes, B. Denizot, G. Tanguy, J. Jacqued, L. Jeune and P. Jallet, J. Colloid Interface Sci. Vol. 212 (1999), p.474.

DOI: 10.1006/jcis.1998.6053

Google Scholar

[3] H.P. Khng, D. Cunliffe, S. Davies, N.A. Turner and E.N. Vulfson, Biotechnlo. Bioeng. Vol. 60 (1998), P. 419.

Google Scholar

[4] S. Gomez-Lopera, R Plaza and A. Delgado, J. Colloid Interface Sci. Vol. 240 (2001), P. 40.

Google Scholar

[5] A. Jordan, R Scholz, P. Wust, H. Fahling and R. Felix, J. Magn. Magn. Mater. Vol. 201 (1999), P. 413.

Google Scholar

[6] M. D Butterworth, L. Illum and S. S. Davis, Colloids surf. A Vol. 179 (2001), P. 93.

Google Scholar

[7] D. V. Szabo and D. Vollath, Adv. Mater. Vol. 11 (1999) P. 1313.

Google Scholar

[8] L. N. Donselaar, A. P. Philipse and Suurmond, J. Langmuir Vol. 13 (1997), P. 6018 1400 1200 1000 800 600 400 200 0 0 5000 10000 15000 20000 25000 30000 N 1s Si 2p Si 2s C 1s O 1s Relative Intensity Binding Energy(eV) $� %�.

Google Scholar