The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles

Abstract:

Article Preview

This work reported the development of the high throughput protein separation process with molecularly assembled silica-coated magnetic nanoparticles as a function of amino group numbers such as mono-, di-, and tri-aminofunctionality, in which the range of silica coating thicknesses were optimized to be interacted with protein. The protein separation efficiency was demonstrated as a function of each aminofunctional group and the particle sizes of the silica coated magnetic nanoparticles. The particles were prepared by the chemical precipitation of Fe2+ and Fe3+ salts with a molar ratio of 1:2 under basic solution. The silica coated magnetic nanoparticles were directly produced by the sol-gel reaction of a tetraethyl orthosilicate (TEOS) precursor, in which the coating layer serves as a biocompatible and versatile group for further biomolecular functionalization. To effectively capture the proteins, silica coated magnetic nanoparticles need to be functionalized reproducibly on the silica surface, and three kinds of amino functional groups were adapted as a function of number of amine using the mono-, di-, and tri-aminopropylalkoxysilanes.

Info:

Periodical:

Solid State Phenomena (Volumes 124-126)

Edited by:

Byung Tae Ahn, Hyeongtag Jeon, Bo Young Hur, Kibae Kim and Jong Wan Park

Pages:

903-906

DOI:

10.4028/www.scientific.net/SSP.124-126.903

Citation:

M. E. Park et al., "The Selective Protein Separations with Polyaminofunctionality on Controlled Silica Coating-Layers of Magnetic Nanoparticles", Solid State Phenomena, Vols. 124-126, pp. 903-906, 2007

Online since:

June 2007

Export:

Price:

$35.00

In order to see related information, you need to Login.

In order to see related information, you need to Login.