H-PDLC Based Electrically Controlled Optical Chopper Applied within the Fluorescence Microscopy System

Ji Hong Zheng; Zeng Jun Zhou; Ping Yu Tang; Yan Meng Jiang

doi:10.4028/www.scientific.net/SSP.181-182.269

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H-PDLC Based Electrically Controlled Optical Chopper Applied within the Fluorescence Microscopy System
p.269

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HomeSolid State PhenomenaSolid State Phenomena Vols. 181-182H-PDLC Based Electrically Controlled Optical...

H-PDLC Based Electrically Controlled Optical Chopper Applied within the Fluorescence Microscopy System

Abstract:

An electrically controlled optical chopper based on holographic polymer dispersed liquid crystal grating (H-PDLC) and its application within the fluorescence microscopy has been investigated in this paper. The H-PDLC chopper has the characteristics such as free waveform, easy and precise tunable working frequency, no moving parts etc. In order to testify its application, a two-channel frequency division multiplexing confocal fluorescence microscopy (FDMCF) has been established, where the integrated H-PDLC chopper array modulates the individual exciting laser beam with different carrying frequency. The FDMCF microscopy detects two-channel fluorescent signals from the two different spots of the specimen’s surface synchronously. The successful experiments testified the optical chopper device based on H-PDLC technique, and explored its application possibility within the modern integrated optical system as well.

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Periodical:

Solid State Phenomena (Volumes 181-182)

Pages:

269-272

DOI:

https://doi.org/10.4028/www.scientific.net/SSP.181-182.269

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Online since:

November 2011

Authors:

Ji Hong Zheng, Zeng Jun Zhou, Ping Yu Tang, Yan Meng Jiang

Keywords:

Fluorescence Confocal Microscopy, Holographic Polymer Dispersed Liquid Crystal, Optical Chopper, Parallel Detection Phase

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