Manganese Removal from Metal Refinery Wastewater Using Mn(II)-Oxidizing Bacteria

Abstract:

There is a growing interest in the use of Mn (II)-oxidizing bacteria to treat Mn-containing metal refinery wastewaters instead of using conventional chemical approaches since the former could reduce the cost of alkaline agents and oxidants to remove Mn (II) as Mn oxides at alkaline pH. The Mn level was found naturally dropped in the industrial metal refinery wastewater treatment system, where the formation of Mn-enriched sludge was apparent. This observation motived us to investigate the possible involvement of microbially mediated reactions. From the sludge sample, Pseudomonas sp. strain SK3 was successfully isolated and tested for its Mn (II)-oxidation characteristics. Strain SK3 completely removed 100 ppm Mn (II) within 42 hours as birnessite ((Na,Ca,K)_0.6(Mn^IV, Mn^III)₂O₄·1.5H₂O) under optimized conditions. Copper ions were found to be an important factor in promoting Mn (II) oxidation. Changes in the Mn (IV)/Mn (III) ratio during bacterial Mn (II)-oxidation indicated the involvement of 2-step one-electron transfer reactions in the formation of biogenic birnessite with Mn (III) as intermediate. Characteristics of strain SK3 were compared with those of a well-known Mn (II)-oxidizing bacterium, Pseudomonas putida strain MnB1. Strain SK3 displayed more robust Mn (II) oxidation capabilities under several severe conditions, showing its ideal characteristics for use in the industrial water treatment process.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 673-676
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.673
• Online since: August 2017
• Authors: Santisak Kitjanukit, Kyohei Takamatsu, Kenji Takeda, Satoshi Asano, Naoko Okibe*
• Keywords: Biogenic Birnessite, Manganese Removal, Mn(II)-Oxidizing Bacteria

References

[1] W. Knocke, J.V.B., M. Kearney, A. Soborski, D. Reckhow, Alternative Oxidants for the Removal of Soluble Iron and Manganese. 1990, Denver, CO: AWWA Reseach Foundation.

[2] L.I. Sly, M.C.H., V. Arunpairojana, Effect of water velocity on the early development of manganese-depositing biofilm in a drinking-water distribution, FEMS Microbiol. Ecol. 53 (1988) 175-186.

DOI: 10.1111/j.1574-6968.1988.tb02662.x

[3] Smith, J.E. and J.D. Oliver, The effects of hydrostatic pressure on bacterial attachment, Biofoul. 3 (1991) 305-310.

[4] Anzai Y., K.H., Park J.Y., Wakabayashi H., Oyaizu H., Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence, Int. J. Syst. Evol. Microbiol., 50 (2000) 1563-1589.

DOI: 10.1099/00207713-50-4-1563

[5] G. Brouwers, E.V., P. Corstjens, E. Jong, Bacterial Mn2+ oxidizing sysmtems and multicopper oxidases: an overview of mechanisms and functions, Geomicrobiol. J. 17 (2000) 1-24.

[6] J. Bargar, S.W., B. Tebo, EXAFS, XANES, and in-situ SR-XRD characterization of biogenic manganese oxides produced in sea water, Physica Scripta. 2005 (2003) 888-890.

DOI: 10.1238/physica.topical.115a00888