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Molecular beacon-based fluorescent biosensor for sensitive detection of single nucleotide polymorphism Songbai Zhang1, a, Meng Tang1, Liying Zheng1, Xia Hu1, Guangyu Shen1, Xuewen Liu1, Jinlin Lu1, Yuandao Chen1, Liping Qiu2 and Shibiao Zhou1 1College of Chemistry and Chemical Engineering, Hunan University of Arts and Science, Changde 415000, PR China 2State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China azsb0119@163.com Keywords: Molecular beacon, structure-switching, fluorescent, biosensor, single nucleotide polymorphism Abstract.
Materials and Methods Chemicals.
Acknowledges This study is financially supported by the National Natural Science Foundation of China (Grant No. 21205039, 21205021, 21105017), Natural Science Foundation of Hunan Province (Grant No. 13JJ6071), Open Project of State Key Laboratory of Chemo/biosensing and Chemometrics (Grant No. 2013004), Doctor’s Start-up Foundation of Hunan University of Arts and Science and the Construct Program of the Key Discipline in Hunan Province (Applied Chemistry).
Yu, Chinese Journal of Chemistry 27 (2009) 523-528

The utilization of METs and AOPs were applied in practical wastewater considering high removal efficiency for man-made wastewater and low removal efficiency for practical wastewater. 2 Materials and methods All reagents used in this experiment were of analytic grade. 1mol/L NaOH and 1mol/L HCl solutions were used to adjust pH value of solution.
Environmental science & technology 34, 591-597 (2000)
Chemical Engineering Journal 148, 342-347 (2009)
Environmental science & technology 26, 2218-2227 (1992)
Environmental science 30. 1425-1429(2009)

Comprehensive Assessment of Heavy Metal Pollution of Urban Surface Soils in Qingdao, China Jinfeng Wang1,a ,Wenbin Zhao2,b, Xianglan Liu2,c,Shouyong Zhang2 1.College of Chemical & Environmental Engineering ,Shandong University of Science and Technology ,Qingdao, Shandong province,China 2.College of Resources & Environmental Engineering,Shandong University of Science and Technology, Qingdao, Shandong province, China awjf0412@163.com, b19762438@163.com, cggxlyf@yahoo.com.cn Keywords: Heavy metals, Geo-accumulation index, Hierarchical cluster analysis, Multidimensional scaling analysis Abstract—Soil samples of 0-5cm from 18 sampling sites including different functional zones of Qingdao, China were collected and analyzed.
Materials and methods Site description and soil sampling.
Maps of sampling sites in Qingdao City: Industrial region(A):A1(Haier industrial region),A2(Licang industrial region),A3(Zhongcun industrial region),A4(Qingdao power plant); Roadside greenbelt(B):B1(Hisense overpass); B2(Hangzhou overpass);Public green space(C):C1 (The May 4th Square);C2(Licun Park);C3(Zhongshan Park);C4(Century Park in chengyang district);C5(Cultural Park in sifang district);C6(Tang Island Bay Park in development zone);Commercial street(D):D1(Taitung walking street);D2(Jusco shopping street in development zone);D3 (Licun commercial street);D4(Hong Kong Mid-Road);School(E):E1(Shandong University of Science and Technology); E2(Qingdao Technological University) Samples treatment.
Acknowledgements The authors express their appreciation for the financial support by Shandong province Natural Science Foundation(ZR2010EQ022), the 47th Postdoctoral foundation of China (20100471563).The authors would like to thank all the persons providing their assistance during sampling and analysis.
Geo-journal 2, 108–118 [5] Qingdao Statistical Bureau, Qingdao Statistical Yearbook, online edition. 2010.Available:http://www.statsqd.gov.cn/statsqd/news/201131110083154862.asp?

The Effect of Clamping Force on Static and Dynamic Characteristics of Spindle-Tooling System Weiguo Wu 1,a, Guicheng Wang 2, Chungen Shen 2, Lijie Ma 2 and Shulin Wang 2 1 Faculty of Science, Jiangsu University, Zhenjiang, China, 212013 2 School of Mechanical Engineering, Jiangsu University, Zhenjiang, China, 212013 awuwg@ujs.edu.cn Keywords: High speed machining, Clamping force, Spindle, Dynamic characteristics Abstract.
Introduction High speed machining (HSM) is used widely in the moulds machining and the aero plane building industry, where large amounts of material are removed from a large structure.
(3) where alim is the limiting axial depth of cut in peripheral end milling operations; Ks the specific cutting force for the work piece material (712×10 6 N/m 2); µxx the 'average' direction orientation factor of the cutting force, and z is the number of cutter teeth (2); N the spindle speed in rpm; m an integer that corresponds to the individual lobe numbers(i.e., N=0 is the right-most lobe, N=1 is first lobe to the left, etc.).
Acknowledges The authors are grateful to National Natural Science Foundation of China (Grant No.50675088)， Natural Science Foundation of Jiangsu province (No.BK2006077) and Senior Qualified Personnel Research Foundation of Jiangsu University for supporting this project. 10.5 kN 19 kN 24 kN References [1] J.
[2] Y.Altintas, S.Engin and E.Budak: Journal of Manufacturing Science and Engineering, Trans.

Precision Conformal Optics Technology Program[C]// Proceedings of SPIE, Window and Dome Technologies and Materials VII, 2001, 4375:96-107
Overview of Conformal Optics[C]// Proceedings of SPIE, Window and Dome Technologies and Materials VI, 1999, 3705:180-188
High Precision Metrology of Domes and Aspheric Optics [C]// Proceedings of SPIE, Window and Dome Technologies and Materials IX, 2005, 5786: 112-121
Magnetorheological Finishing of IR Materials[J]// Proceedings of SPIE, Optical Manufacturing and Testing Ⅱ, 1997, 3134: 258-269
Magnetorheological Finishing Tool and Removal Function[J]// Journal of Tsinghua University (Science & Technology), 2004, 44(2): 190-193

The semiconductor industry is looking for different materials and devices to integrate with the current silicon based technology in a long term future.
CNT’s have unique properties such as stiffness, strength, and tenacity compared to other materials especially to silicon [13].
Polysilicon have been utilized as gate materials since the evolution of MOSFET device.
Drain current vs. drain voltage for different High-K materials Drain voltage at fixed drain current for different High-K materials are shown in plot of Fig.8.
It is very much clear that in CNTFET device, at High-K materials the requirement of drain voltage is very less for a particular drain current.

Removal of Heavy Metals from Contaminated Soils by Washing with Citric Acid and Subsequent Treatment of Soil-washing Solutions Lei SUN1, Han-qiao LIU1,2,3*, Guo-xia WEI1, Zhen-hua WU1, Wei YANG2 1School of Energy and safety Engineering, Tianjin Chengjian University, 300384, Tianjin, China 2Tianjin Technology & Engineering Center of Contaminated Sites Remediation, 300468, Tianjin, China 3School of Environment science and Technology, Tianjin University, 300072, Tianjin, China *lhqlkx@aliyun.com Keywords: heavy metals; contaminated soil; washing; adsorption Abstract: Soil washing experiments were carried out with citric acid as washing reagent for the remediation of soils highly contaminated with heavy metals, then activated carbon was used in absorption processing for leaching solution.
Materials and Methods Soil Sample Preparation Soil samples were collected from the greenbelt in Tianjin Chengjian University, which were measured as weak acid by pH meter.
Journal of Hazardous Materials, 2001, 85: 111-125
Journal of Hazardous Materials, 2008, 154: 153-160.

Applied Mechanics and Materials.852:459-65
Journal of Pipeline Systems Engineering and Practice. 14(3):03123001
Materials Today: Proceedings. 80:1150-7
Materials Today: Proceedings. 26:781-6
Corrosion Science. 85:318-30.

Materials and methods Materials.
Journal of Hazardous Materials. 239-240, 348-354(2012) [5] J.
Ozone: Science & Engineering: The Journal of the International Ozone Association. 35:6, 438-447. (2013)

Materials and Experimental Details Materials The component of grate bars is as follow (wt %): C: 1.9, Si: 1.2, Mn: 0.99, P: 0.019, S: 0.019, Cr: 26.2, Ni: 0.74.
Materials Science and Engineering A, Vol. 406 (1)(2005), p. 217 [9] Ö.Doğan, J.
Zhang.Materials for Mechanical Engineering, Vol. 5(2006), p.78 [11] S.Y.
Journal of Yancheng Institute of Technology Natural Science Edition, Vol. 21 (1)(2008), p.62 [12] H.G.
Transactions of Materials and Heat Treatment, Vol. 26 (2) (2005), p.55