Molecular Beacon-Based Fluorescent Biosensor for Sensitive Detection of Single Nucleotide Polymorphism

Abstract:

A sensitive fluorescent biosensing strategy based on structure-switching of molecular beacon for the detection of single nucleotide polymorphism is proposed in this study. In the absence of target DNA, only low background fluorescence can be detected since the formation of hairpin structure induces close proximity of fluorophore and quencher. On the contrary, the fluorescence intensity increases significantly after introduction of target DNA because structure-switching of molecular beacon results in separation of fluorophore and quencher. The change of fluorescence intensity is linear with concentration of target DNA, and a molecular beacon-based sensing system for fluorescent detection of single nucleotide polymorphism is fabricated. The target DNA can be sensitively detected in a linear dynamic range from 17.58nM-1.125μM with a low detection limit of 8 nM. Moreover, good reproducibility and high specificity are achieved.

• Periodical: Applied Mechanics and Materials (Volumes 651-653)
• Pages: 256-259
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.651-653.256
• Online since: September 2014
• Authors: Song Bai Zhang*, Meng Tang, Li Ying Zheng, Xia Hu, Guan Gyu Shen, Xue Wen Liu, Jin Lin Lu, Yuan Dao Chen, Li Ping Qiu, Shi Biao Zhou
• Keywords: Biosensor, Fluorescent, Molecular Beacon, Single Nucleotide Polymorphisms, Structure-Switching

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