In Situ Pollen Germination and Artificial Pollination Compatibility in Cassava (Manihot esculenta Crantz)

Abstract:

Cassava cultivars are self-compatible, sufficient pollination and fertilization are important factors affecting the rate of fruit set and fruit quality, but the effects of compatible pollination relationships on cassava pollen development and fruit set are poorly understood. In the present study, in situ pollen germination and compatible relationship were investigated by using self-pollination and cross-pollination between two cassava cultivars (SC5 and SC7). The observation in situ pollen germination was carried out with toluidine blue staining method under the fluorescence microscope. The result shows that after self-pollination for 20 min, the pollens, released from SC5 anthers, started to produce pollen tubes and the maximum germination rate (GR) was 39.2%. It cost 60 min for the pollen tubes carrying sperm cells to penetrate through the pistil extracellular matrices of the transmitting tract to the ovary. However, after cross-pollination for 10 min, the pollens started to germinate and maximum GR was 66.8%. It took 30 min for the pollen tube trip to reach ovary. Additionally, the analysis of fruit set indicated that pollination compatibility in cross-pollination was significantly higher than that in self-pollination. This work provided cassava cross breeding a clue that foreign pollen may facilitate fertilization and increase fruit set.

• Periodical: Applied Mechanics and Materials (Volumes 651-653)
• Pages: 245-251
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.651-653.245
• Online since: September 2014
• Authors: Hang Gui Lai, Xia Chen, Zheng Chen, Ya Qiu Zhou, Wen Jun Ou, Kai Mian Li, Jian Qiu Ye, Song Bi Chen*
• Keywords: Cassava, Fruit Set, Pollen Germination, Pollination Compatibility

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