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Genetic Toxicity of Berberine on Mouse Heart

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Abstract:

Berberine, a common drug, which is widely used in clinical medicine as inflammatory medicines, is rarely studied about its affection on non-target organisms in recent researches. To evaluate the eco-toxicity of berberine in mouse heart cells, biochemical responses including oxidative damage and changes in the activities of antioxidative enzymes catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and malondialdehyde (MDA) induced by berberine were investigated in mouse heart cells. 7.5, 15, 30, 60 and 120 mgkg-1 berberine was added to mouse heart cells respectively. Compared to the controls, the damage, tail DNA content (%) , tail length and tail moment went up respectively in dose-effect manner following the rising berberine; As the content of berberine increased, the activities of SOD, CAT and POD were gradually decreased and the concentration of MDA was gradually increased. Compared to the controls, the change tendency showed significant differences (p<0.05 or p<0.01). In conclusion, berberine induces oxidative stress and DNA damage to heart cells, which may be the significant mechanisms for its toxicity to mice.

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Periodical:

Advanced Materials Research (Volumes 884-885)

Pages:

634-637

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.884-885.634

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Online since:

January 2014

Authors:

Cheng Bin Xu, Lu Wang, Xiu Juan Hui, Xi Ping Ma, Xin Bi, Quan Zhao

Keywords:

Berberine, Comet Assay, Genotoxicity, Heart Cells, Oxidative Damage

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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