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Expression Patterns of Cell Surface Molecules on Human Bone Marrow Stromal Cells from Multi-Donors
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Mechanical Properties of Living Cells: Interaction of the Adherent Endothelial Cell and the Fluid Shear Flow
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Preliminary Study for Evaluating Bone Forming Ability of Porous Bioceramics Using Rat Mesenchymal Stem Cells to be Used for International Standard
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Reconstruction of Liver Organoid Using an Apatite-Fiber Scaffold, a Radial-Flow Bioreactor and FCL-4 Cells of Hepatocyte Model
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Preliminary Study for Evaluating Bone Forming Ability of Porous Bioceramics Using Rat Mesenchymal Stem Cells to be Used for International Standard

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Abstract:

In the field of tissue engineering for bone regeneration, there have been many studies that examined in the bone forming ability of the porous biomaterials with mesenchymal stem cells (MSCs). To promote the tissue engineering approach in clinical situation, there is a need for the establishment and standardization of evaluation methods for measuring the in vivo bone forming ability. In this study, we examined the seeding process using rat MSCs to ascertain whether it is a valid protocol for various materials. Our results showed that the cell seeding process for the fabrication method of MSCs/materials composite influenced the number and distribution of the MSCs in the materials, therefore the process is a key to show new bone formation which derived from the seeded MSCs. Here, we describe the detailed process which can show consistent new bone formation in pores of the materials.

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Periodical:

Key Engineering Materials (Volumes 361-363)

Pages:

1161-1164

DOI:

https://doi.org/10.4028/www.scientific.net/KEM.361-363.1161

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Online since:

November 2007

Authors:

Mika Tadokoro, Motohiro Hirose, Hajime Ohgushi

Keywords:

In Vivo Bone Forming Ability, Mesenchymal Stem Cell (MSC), Porous Material, Standard Method

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© 2008 Trans Tech Publications Ltd. All Rights Reserved

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