Optimization of Poly(β-L-malic acid) Production Using Aureobasidium Pullulans by Response Surface Methodology

Chang Sheng Qiao; Kun Zhong; Hua Xuan Hao; Yuan Yuan Jia

doi:10.4028/www.scientific.net/AMM.108.121

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 108Optimization of Poly(β-L-malic acid) Production...

Optimization of Poly(β-L-malic acid) Production Using Aureobasidium Pullulans by Response Surface Methodology

Abstract:

Plackett-burman (P-B) and response surface methodology (RSM) were used to optimize the conditions of Poly (β-L-malic acid) production by Aureobasidium pullulans. Results of the statistical analysis showed that the concentration of Poly (β-L-malic acid ) was influenced by the concentrations of initial glucose, (NH₄)₂SO₄, CaCO₃ and agitation speed, liquid loading, and incubation time. The optimized fermentation conditions performed in shaking flasks were glucose 100.428g/L, (NH₄)₂SO₄ 3.06g/L, succinic acid 2g/L, K₂CO₃ 0.4g/L, KH₂PO₄ 0.1g/L, CaCO₃ 27.7g/L, ZnSO₄·7H₂O 5×10-5g/L, MgSO₄·7H₂O 0.1g/L, and corn steep liquor 0.1% (v/v), liquid loading 55mL/500mL, temperature 25°C, agitation speed 207 r/min, and incubation time 152h. Under which the maximum Poly (β-L-malic acid) concentration of 3.897±0.472g/L was obtained at the optimum conditions, which significantly increased by 218.8% compared with the control (1.781±0.342 g/L).

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Applied Mechanics and Materials (Volume 108)

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121-126

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.108.121

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October 2011

Authors:

Chang Sheng Qiao, Kun Zhong, Hua Xuan Hao, Yuan Yuan Jia

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Aureobasidium Pullulans, Optimization, Poly (β-L-Malic Acid), Response Surface Method (RSM)

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