Based on the CTOD test, metallographic test is done. The effect of microstructure on CTOD of welded joints for offshore high-strength steel has been analyzed by SEM. The microstructure of different samples in a welding procedure and two different welding procedures are compared. From the microscopic point of view, the reasons why the toughness of the second group welded joints is better than the first group’s and why the toughness of the same group welded joints is different have been determined. And the main microstructure found in EQ70 steel welded joints is the ferrite, tempered sorbite and a small amount of pearlite. Besides some carbide particles are on the matrix of tempered sorbite. The material toughness with a small amount of acicular ferrite is better. Inclusions and pores also seriously affect the toughness of welded joints. Reducing the welding heat input appropriately can refine the grain of CGHAZ and improve toughness. But hardened microstructure may occur owing to too low welding heat input.