A wireless temperature monitoring system based on fluorescence lifetime is researched in this paper, which mainly consists of the temperature acquisition unit and the master control monitoring unit. The wireless module with the core of low-power microcontroller (SX1212) and the sensor based on optical-fiber fluorescence are designed. And the unit of fluorescence temperature measurement is introduced in detail. Testing result indicates that the communication distance of the wireless module can be up to 300 meters, which meets the requirements very well. There are many advantages in this system, such as no wiring, better scalability, real-time monitoring.