Cloning and Expression of TLR4-EGFP Fusion Protein in HT-29 Cells and the Visualized Interaction between TLR4 and Enteropathogenic E.coli
The aim of this study was to determine whether the Escherichia coli (EPEC) attached to cells by the TLR4. A fusion between gene encoding fluoresce-enhanced green fluorescent protein (EGFP) and Toll like receptor 4 (TLR4) was constructed and expressed in Escherichia coli. Then the pEGFP-N1-TLR4 fusion expression vector was transfected into HT-29 cells. The green fluoresce was observed at the plasma membrane of HT-29 cells under inverted fluorescent microscopes. In addition, the FITC stained EPEC was also observed adherence to the TLR4 of HT-29 cells.The results indicated that the EPEC adhered to cells by bonding to TLR4 protein. It is the first time that the TLR4 has been explained as an adhesion receptor.
Q. X. Gao et al., "Cloning and Expression of TLR4-EGFP Fusion Protein in HT-29 Cells and the Visualized Interaction between TLR4 and Enteropathogenic E.coli", Applied Mechanics and Materials, Vols. 138-139, pp. 1168-1173, 2012