Cloning and Expression of TLR4-EGFP Fusion Protein in HT-29 Cells and the Visualized Interaction between TLR4 and Enteropathogenic E.coli

Quan Xin Gao; Li Li Qi; Jin Bo Wang; Qing Cha Zhuang; Shi Jian Chen; Xiang Xu; Zhi Zheng

doi:10.4028/www.scientific.net/AMM.138-139.1168

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 138-139Cloning and Expression of TLR4-EGFP Fusion Protein...

Cloning and Expression of TLR4-EGFP Fusion Protein in HT-29 Cells and the Visualized Interaction between TLR4 and Enteropathogenic E.coli

Abstract:

The aim of this study was to determine whether the Escherichia coli (EPEC) attached to cells by the TLR4. A fusion between gene encoding fluoresce-enhanced green fluorescent protein (EGFP) and Toll like receptor 4 (TLR4) was constructed and expressed in Escherichia coli. Then the pEGFP-N1-TLR4 fusion expression vector was transfected into HT-29 cells. The green fluoresce was observed at the plasma membrane of HT-29 cells under inverted fluorescent microscopes. In addition, the FITC stained EPEC was also observed adherence to the TLR4 of HT-29 cells.The results indicated that the EPEC adhered to cells by bonding to TLR4 protein. It is the first time that the TLR4 has been explained as an adhesion receptor.

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Applied Mechanics and Materials (Volumes 138-139)

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1168-1173

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https://doi.org/10.4028/www.scientific.net/AMM.138-139.1168

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Online since:

November 2011

Authors:

Quan Xin Gao, Li Li Qi, Jin Bo Wang, Qing Cha Zhuang, Shi Jian Chen, Xiang Xu, Zhi Zheng

Keywords:

E.coli, EGFP, Fusion Protein, HT-29 Cells, TLR4

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