Cloning and Expression of TLR4-EGFP Fusion Protein in HT-29 Cells and the Visualized Interaction between TLR4 and Enteropathogenic E.coli

Abstract:

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The aim of this study was to determine whether the Escherichia coli (EPEC) attached to cells by the TLR4. A fusion between gene encoding fluoresce-enhanced green fluorescent protein (EGFP) and Toll like receptor 4 (TLR4) was constructed and expressed in Escherichia coli. Then the pEGFP-N1-TLR4 fusion expression vector was transfected into HT-29 cells. The green fluoresce was observed at the plasma membrane of HT-29 cells under inverted fluorescent microscopes. In addition, the FITC stained EPEC was also observed adherence to the TLR4 of HT-29 cells.The results indicated that the EPEC adhered to cells by bonding to TLR4 protein. It is the first time that the TLR4 has been explained as an adhesion receptor.

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Periodical:

Edited by:

Honghua Tan

Pages:

1168-1173

DOI:

10.4028/www.scientific.net/AMM.138-139.1168

Citation:

Q. X. Gao et al., "Cloning and Expression of TLR4-EGFP Fusion Protein in HT-29 Cells and the Visualized Interaction between TLR4 and Enteropathogenic E.coli", Applied Mechanics and Materials, Vols. 138-139, pp. 1168-1173, 2012

Online since:

November 2011

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$35.00

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