Research the Equilibrium Distribution of Puerarin in Aqueous Two-Phase Combine with Temperature-Induced Phase Separation EOPO/ K2HPO4 /Water Systems

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The partition behavior of Puerarin in ethylene oxide-propylene oxide random copolymer (EOPO)/ K2HPO4/water systems was investigated .The optimum conditions were EOPO (SDP30) 28%, K2HPO4 24%, temperature-induced condition was 70°C and 30min. yield of Puerarin could reach 42.26%. Based on the modified model lnK/R2=A*+b*(w”-w’)+c*(w”-w’)2, partition coefficients of Puerarin in EOPO/K2HPO4 aqueous two-phase systems were correlated. It was found that the correlation of the experimental data was quite satisfactory.

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1162-1167

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November 2011

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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