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Chemical Constituent and Antimicrobial Activity of Essential Oil from Fragrant Loquat and Common Loquat
Abstract:
The chemical components of fragrant loquat and common loquat extracted by petroleum ether had been investigated using capillary GC-MS. 109 constituents were identified and the relative percentages of the constituents were determined. The highest content of chemical constituents was phytol. The antimicrobial activity on seven test organisms of essential oil from fragrant loquat and common loquat leaf extract were detected, the results indicated that these extracts had inhibitory effects on Canidia Albicans and Trichoderma spp., but not on bacteria.
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Periodical:
Pages:
974-980
Citation:
Online since:
November 2011
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Keywords:
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Сopyright:
© 2012 Trans Tech Publications Ltd. All Rights Reserved
Citation:
[1] S. Lin, World loquat production and research with special reference to China, Acta Hort., vol. 750, pp.37-44, April (2007).
[2] Y. Wang, Y. Zhou, J. Zhang, Pharmacodynamics of arbutin on relieving cough, dispelling phlegm and preventing asthma, Chin Tradi Herb Drugs , vol. 34, no. 3, pp.739-741, March (2003).
[3] X. Yang, S. Lin, New ideas on the classification of loquat, South China Fruits, vol. 36, p.28–31, May (2007).
[4] Y. Hong, S. Lin, and X. Huang, Determination of Ursolic acid in Eriobotrya Leaves and Terpenoid Fingerprinting, Acta Hort., vol. 750, pp.225-232, April (2007).
[5] Editorial Office of National Chinese Herb Medicine Collection, Collection of National Chinese Herbal Medicine, " Eds. Beijing: People, s Medical Publishing House, 1975, pp.491-492.
[6] R. Tai, X. Xu, W. Guo, Analysis of chemical composition of essential oil in leaves of Eriobotrya japonica by GC-MS, Chin Hosp Pharm J, vol. 28, no. 3, pp.206-208, Feb. (2008).
[7] Y. Zhou, L. Qian, C. Huang, Identification of Antibacterial Activity of Plant Extract by A Colormetric Detection Method, Food Science, vol. 29, no. 5, pp.140-141, May (2008).
[8] Z. Liu, J. He, Z. Yu, Analysis of Volatile Components of Alfalfa by Gas Chromatography-Mass pectrometry (GC/MS), Chem. Bioengi., vol. 23, no. 4, pp.60-62, April (2006).
[9] Das B, Yeger H, Baruchel H, Freedman M, Koren G, and Baruchel S. In vitro cytoprotective activity of squalene on a bone marrow versus neuroblastoma model of cisplatin-induced toxicity: implications in cancer chemotherapy, Eur. J. Cancer, vol. 39, no. 17, pp.2556-2565, Nov. (2003).
[10] Y. Hong, S. Lin, Y. Jiang, M. Ashraf, Variation in contents of total phenolics and flavonoids and antioxidant activities in the leaves of 11 Eriobotrya Species, Plant Foods Hum. Nutr., vol. 63, no. 4, pp.200-204.
[5] 27 C7H16O 3-Hexanol, 3-methyl.
24.
5 2.
[5] 61 C6H12O 3-Hexen-1-ol.
3 3.
[5] 83 C6H14O 1-Hexanol.
[1] 19.
3 4.
[6] 26 C6H14O 1-Pentanol, 4-methyl.
07.
07 5.
[6] 67 C5H10O2 Butanoic, 2-methyl.
38.
08 6.
[7] 43 C5H10O2 Pentanoic acid.
04 7.
[8] 37 C7H16O 1-Pentanol, 2, 3-dimethyl.
03.
05 8.
[10] 35 C7H16O 1-Heptanol.
03 9.
[10] 66 C8H14O 5-Hepten-2-one, 6-methyl.
02 10.
[12] 08 C6H12O2 Hexanoic acid.
[4] 28.
67.
[1] 99 11.
[17] 53 C6H10O2 3-Hexenoic acid.
13.
15 12.
[17] 8 C10H18O2 cis-Linaloloxide.
25 13.
[17] 81 C8H8O2 Benzoic acid, methyl ester.
06.
19 14.
[18] 43 C9H18O 1-Nonen-4-ol.
[2] 87.
26 15.
[18] 87 C8H16O 1-Methylcycloheptanol.
46.
13 16.
[19] 18 C10H18O Linalool.
74.
29.
17 17.
[19] 69 C11H24 Undecane.
24.
08 18.
[20] 01 C11H20 1-Methyl decahydronaphthalene.
04 19.
[20] 48 C10H18O p-Menth-8-en-3-ol.
19.
1 20.
[20] 8 C10H18O (-)Isopulegol.
03 21.
[20] 97 C9H10O2 Benzeneacetic acid methyl.
02 22.
[21] 22 C9H18O 6-Nonen-1-ol.
51.
18.
1 23.
[21] 43 C9H20O 1-Nonanol.
08 24.
[21] 47 C10H22O 3, 4, 5-Trimethyl-4-heptanol.
[1] 08.
29 25.
[21] 68 C10H18O Terpineol.
29.
17 26.
[21] 81 C10H20O2 Hexylbutyrate.
64.
87.
15 27.
[21] 84 C7H5NS Benzothiazole.
2 28.
[22] 06 C10H16O α-Cyclocitral.
06 29.
[22] 12 C10H18O2 Bicyclo[3. 1. 0]hexane-6-menthanol-2- hydroxy-1, 4, 4-trimethyl.
66.
16 30.
[22] 3 C12H26 Dodecane.
77.
24.
31 31.
[22] 58 C10H18O 2, 6-Octadien-1-ol-3, 7-dimethyl.
36 32.
[22] 62 C13H26O Tetrahydroionol.
22 33.
[22] 66 C13H28 Undecan, 2, 6-dimethyl.
04 34.
[22] 8 C11H22O2 Butanoic acid, 2-methyl-, hexyl ester.
24.
18.
06 35.
[22] 98 C12H24 Cyclohexan, hexyl.
05 36.
[23] 08 C10H18O 1, 6-Octadien-3-ol, 3, 7-dimethyl.
3 37.
[23] 1 C12H20O2 linalyl acetate.
18 38.
[23] 13 C11H20O 1, 7-Nonadien-4-ol, 4, 8-dimethyl.
68.
2 39.
[23] 68 C12H20O2 Nerol acetate.
37 40.
[23] 75 C11H24 2, 3, 7-Trimethyl Octan.
07 41.
[23] 77 C14H30O 1-Tetradecane.
[1] 35.
3 42.
[24] 16 C13H28 Tridecan.
88.
18.
27 43.
[24] 39 C9H16O2 2(3H)-Furanone, dihydro-5-pentyl.
82.
08.
27 44.
[25] 17 C10H16O2 p-Menth-2-ene, 1, 4-epidoxy.
97.
23 45.
[25] 27 C12H24O2 Hexanoic acid, hexyl ester.
[1] 3.
36 46.
[25] 41 C15H32 Tetradecane, 2, 6, 10-trimethyl.
39.
11.
09 47.
[25] 51 C14H28O Tetradecanal.
11 48.
[25] 56 C16H34O 1-Hexadecanol.
04 49.
[25] 68 C14H30 Tetradecane.
84.
28.
4 50.
[25] 75 C13H20O α-Ionone.
44.
19.
18 51.
[26] 01 C15H28 Decahydro-4, 4, 8, 9, 1-pentamethyl.
06.
04 52.
[26] 07 C13H22O Neryl acetone.
49.
42.
3 53.
[26] 12 C15H24 2-Norpinene, 2, 1-dimethyl-6-(4-mrthyl-3-, pentenyl).
12 54.
[26] 31 C14H20O2 p-Benzoquinone, 2, 6, di-tert-butyl.
11 55.
[26] 43 C15H24O2 6-Di(t-butyl)-4-hydroxy-4-methyl-2, 5- cyclohexadien-1-one.
25.
16 56.
[26] 49 C12H20O2 α-Ionon-5, 6-epoxide.
55.
31 57.
[26] 54 C13H20O β-Ionone.
[2] 15.
54.
59 58.
[26] 64 C11H22O2 1, 1-Diethoxy-2-heptene.
18.
16 59.
[26] 82 C11H16O2 Dihydroactinidiolide.
[2] 78.
58.
[1] 34 60.
[26] 9 C14H22O Phenol, 2, 4-di-tert-butyl.
14 61.
[27] 02 C15H32 Pentadecane.
24.
26 62.
[27] 61 C15H26O trans-Nerolidol.
[1] 05.
[1] 1.
71 63.
[27] 9 C15H24O Caryophyllene oxide.
15.
19.
1 64.
[28] 15 C15H26O Cedrenol.
18.
19.
12 65.
[28] 25 C15H24O2 Limonen-6-ol, pivalate.
56 66.
[28] 28 C16H34 Hexadecane.
15 67.
[28] 34 C17H28O4 Nerolidol-epoxyacetate.
27 68.
[28] 37 C13H20O2 4-Hydro-α-ionone.
37 69.
[28] 5 C13H20O2 3-Oxo-α-ionol.
2 70.
[28] 57 C15H26O cadinol.
05 71.
[28] 89 C15H24O4 2-Acetoxy-1, 1, 10-trimethyl-6, 9-epidioxydecalin.
45.
32 72.
[28] 92 C17H36O 1-Hexadecanol, 2-methyl.
37 73.
[28] 97 C13H22O3 2-Hydroxy-1, 1, 10-trimethyl-6, 9-epidioxydecanlin.
35.
37 74.
[29] 38 C17H36 Tetradecane, 2, 6, 10-trimethyl.
33.
48.
18 75.
[29] 5 C19H40 Pentadecan, 2, 6, 10, 14-tetramethyl.
19.
23 76.
[29] 61 C11H16O3 (-)-Lolilide.
29 77.
[29] 75 C14H18O3 7-Menthoxy-5-ethoxy-2, 2-dimethyl-2H-chromene.
54 78.
[29] 77 C20H40 2-Hexadecene, 3, 7, 11, 15-tetramethyl.
19.
09 79.
[30] 47 C17H36 Heptdecane.
06.
11.
05 80.
[30] 67 C15H23NO3 N-(3, 5-di-tert-butyl-4-hydroxyphenyl)- Carbanmic acid.
06 81.
[30] 78 C18H36O 2-Pentadecanone, 6, 10, 14-trimethyl.
[1] 67.
[1] 34.
[2] 33 82.
[30] 85 C20H38 Neophytadiene.
[1] 27.
[4] 42.
16 83.
[31] 31 C16H22O4 Phthalic acid, disobutyl ester.
07 84.
[31] 28 C20H40O 3, 7, 11, 15-Tetramethyl-2-hexadecen-1-ol.
56.
[1] 77.
27 85.
[31] 43 C18H30O Farnesyl acetone.
18.
14.
2 86.
[31] 57 C17H34O2 Methyl palmitate.
[1] 06.
52.
[1] 06 87.
[31] 67 C16H22O4 Dibutyl phthalate.
12.
11.
84 88.
[31] 88 C20H40O Isophytol.
82.
23 89.
[32] 46 C19H40 Nonadecane.
08 90.
[32] 58 C20H34O Geranyl linalool isomer.
27.
64.
29 91.
[33] 14 C19H34O2 Linoleic acid, methyl ester.
81.
[1] 53 92.
[33] 18 C20H36O2 Linoleic acid, ethyl ester.
13 93.
[33] 23 C19H36O2 Oleic acid, methyl ester.
65.
[1] 56 94.
[33] 46 C20H40O Phytol 27.
[57] 44.
[17] 81 95.
[33] 76 C20H41O Phytol isomer.
64 96.
[33] 86 C19H22O6 Isochiapin B.
55 97.
[33] 92 C17H30O2 Hexadecandienoic acid methyl ester.
[2] 32 98.
[34] 29 C20H42 Eicosan.
15 99.
[35] 15 C21H44 Heneicosan.
17 100.
[35] 45 C21H40O2 4, 8, 12, 16-Tetramethylheptadecan-4-olide.
73.
[1] 28 101.
[37] 23 C29H50O Clionasterol.
[2] 54.
[12] 26 102.
[37] 35 C24H38O4 Bis(2-ethylhexyl)Phthalate.
32.
49.
[1] 11 103.
[38] 22 C20H40O 3, 7, 11, 15-Tetramethyl-2-hexadecan-1-ol.
[1] 01 104.
[39] 03 C17H28O2 8αH-Cedran-8-ol, acetate.
22.
08 105.
[40] 56 C29H50O2 Vitamin E.
[1] 36.
[4] 14 106.
[41] 37 C30H50O Lupeol.
[1] 23 107.
[42] 54 C31H50O3 Methyl-3a-hydroxyolean-12-en-28-oate.
[2] 55.
[16] 87 108.
[42] 64 C28H56 Octacosane.
[2] 65 109.
[42] 92 C30H50 Squalene 5.
[3] 36 TABLE Ⅱ The antimicrobial activity of petroleum ether extract of fragrant loquat and common loquat Microorganism antimicrobial circle diameter（mm） MIC（mg/mL） Common loquat Fragrant loquat Common loquat Fragrant loquat Potassium sorbate Staphylococcus aureus - - - - 40 E. coli - - - - 40 Salmonella - - - - 40 P. aeruginosa - - - - 50 Proteus vulgaris - - - - 50 Canidia Albicans 10 11 10 10 80 Trichoderma spp. 15 9.
313 20 30.