Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System
In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.
W. J. Wang et al., "Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System", Applied Mechanics and Materials, Vols. 138-139, pp. 995-1001, 2012