Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

Abstract:

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

• Periodical: Applied Mechanics and Materials (Volumes 138-139)
• Pages: 995-1001
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.138-139.995
• Online since: November 2011
• Authors: Wei Jie Wang, Xiao Le Zhang, Chang Bo Xu, Hong Yan Cheng
• Keywords: Aqueous Two-Phase Extraction, C-Phycocyanin, PEG-2000, Potassium Sodium Tartrate, Purification

References

[1] P. J. Viskari, C. L. Colyer, Rapid extraction of phycobiliproteins from cultured cyanobacteria samples, Anal. Biochem., vol. 319, p.263–271, (2003).

DOI: 10.1016/s0003-2697(03)00294-x

[2] S. T. Silveira, J.F.M. Burkert, et al. Separation of phycocyanin from Spirulina platensis using ion exchange chromatography, Bioresource Technology, vol. 98, p.1629–1634, (2007).

DOI: 10.1016/j.biortech.2006.05.050

[3] A. P. Batista, A. Raymundo, I. Sousa, J. Empis, Rheological characterization of coloured oil-in-water food emulsions with lutein and phycocyanin added to the oil and aqueous phases, Food Hydrocolloids, vol. 20, pp.44-52, (2006).

DOI: 10.1016/j.foodhyd.2005.02.009

[4] M. Shahbaz, Hamid, Development and Application of Aqueous Two-Phase Partition for the Recovery and Separation of Recombinant Phenylalanine Dehydrogenase, Iran. J. Chem. Chem. Eng., vol. 27, pp.119-127, (2007).

[5] A. Bennett, L. Bogorad. Complimentary chromatic adaptation in a filamentous blue-green alga, J. Cell Biol. vol. 58, p.419–435, (1973).

DOI: 10.1083/jcb.58.2.419

[6] S. Tanuja, N. D. Srinivas, Aqueous two-phase extraction for downstream processing of amyloglucosidase, Process Biochemistry, vol. 32, pp.635-641, (1997).

DOI: 10.1016/s0032-9592(97)00009-5

[7] S. Chethana, C. A. Nayak, K.S.M.S. Raghavarao, Aqueous two phase extraction for purification and concentration of betalains, Journal of Food Engineering, vol. 81, p.679–687, (2007).

DOI: 10.1016/j.jfoodeng.2006.12.021

[8] G. Patil, K.S.M.S. Raghavarao, Aqueous two phase extraction for purification of C-phycocyanin, Biochemical Engineering Journal, vol. 34, p.156–164, (2007).

DOI: 10.1016/j.bej.2006.11.026

[9] P.A. Albertsson, Partition of Cell Particles and Macromolecules, 2nd ed., Wiley, New York, (1986).

[10] Hatti-Kaul. Rajni, Aqueous Two-Phase Systems Methods and Protocols, Humana Press Inc., Totowa, New Jersey, (1999).