In this work, Antheraea pernyi silk fibroin (ASF) /chitosan (CS) blend scaffolds were prepared by freeze-drying. The relationship between preparation conditions and morphological structures of blend scaffolds was also studied. The results indicated that the porosity and pore diameter of the ASF /CS scaffolds decreased with increasing of the proportion of chitosan and decreasing of the freezing temperature. By changing the process conditions, the average pore diameter and porosity of ASF/CS scaffolds could be adjusted in the range of 134 - 527 μm and 71 - 91% respectively. Compared with the pure ASF or CS materials, the ASF / CS (60/40) blend materials were more favor of cell adhesion.