Investigations of the Effects of Epigallocatechin-3-Gallate and Co2+ on Cal-27 Cells In Vitro

Abstract:

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Epigallocatechin gallate (EGCG), a major polyphenol of green tea, has been shown to inhibit the growth of various cancer cell lines. Co2+ is one of essential trace elements of human nutrition, both lack and excess have adverse effects on health.In this research, MTT assays were applied to investigate the effects of Co2+, EGCG and their coactions on Cal-27 cells, The results show that Co2+ and EGCG inhibited the growth of Cal-27 cells in a time-and dose-dependent model, and inhibition effects were extremely dependent on their concentrations, added orders and [Co2+]/[EGCG] ratio. Inhibitory effects of Co2+ could be decreased in the presence of EGCG. LC/MS analysis showed that a new component arised when EGCG and Co2+ were mixed together. It was inferred that the new components were [EGCG-2Co] and [EGCG-Co], and the process might be responsible for the observed effect of the bioactivities of Co2+ against Cal-27 cells.

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Periodical:

Edited by:

Robert Zhu

Pages:

167-171

DOI:

10.4028/www.scientific.net/AMM.140.167

Citation:

J. S. Su et al., "Investigations of the Effects of Epigallocatechin-3-Gallate and Co2+ on Cal-27 Cells In Vitro", Applied Mechanics and Materials, Vol. 140, pp. 167-171, 2012

Online since:

November 2011

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$35.00

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