Investigations of the Effects of Epigallocatechin-3-Gallate and Co2+ on Cal-27 Cells In Vitro

Jian Sheng Su; Wen Fei Han; Hai Bo Wang

doi:10.4028/www.scientific.net/AMM.140.167

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 140Investigations of the Effects of...

Investigations of the Effects of Epigallocatechin-3-Gallate and Co²⁺ on Cal-27 Cells In Vitro

Abstract:

Epigallocatechin gallate (EGCG), a major polyphenol of green tea, has been shown to inhibit the growth of various cancer cell lines. Co²⁺ is one of essential trace elements of human nutrition, both lack and excess have adverse effects on health.In this research, MTT assays were applied to investigate the effects of Co²⁺, EGCG and their coactions on Cal-27 cells, The results show that Co²⁺ and EGCG inhibited the growth of Cal-27 cells in a time-and dose-dependent model, and inhibition effects were extremely dependent on their concentrations, added orders and [Co²⁺]/[EGCG] ratio. Inhibitory effects of Co²⁺ could be decreased in the presence of EGCG. LC/MS analysis showed that a new component arised when EGCG and Co²⁺ were mixed together. It was inferred that the new components were [EGCG-2Co] and [EGCG-Co], and the process might be responsible for the observed effect of the bioactivities of Co²⁺ against Cal-27 cells.

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Applied Mechanics and Materials (Volume 140)

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167-171

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https://doi.org/10.4028/www.scientific.net/AMM.140.167

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November 2011

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Jian Sheng Su, Wen Fei Han, Hai Bo Wang

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Cal-27 Cells, Co²⁺, EGCG, Green Tea

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