Preparation, Partial Isolation of Antifreeze Peptides from Fish Gelatin with Hypothermia Protection Activity

Abstract:

Antifreeze proteins have characteristics of inhibiting the growth of crystals, decreasing the injury of cells and can retain the structure, texture and quality of productions. The purpose of this study is to obtain natural antifreeze peptides, and to investigate the hypothermia protection activity on bacteria. Gelatin derived from shark skin was hydrolysed to obtain antifreeze peptides. The most appropriate protease and hydrolysis time was selected with the index of the hypothermia protection activity on bacteria. The hydrolysate was subsequently added on to Sephadex G-50 gel filtration column and SP-Sephadex C-25 column to acquire high activity fractions. The fraction of cationic peptides termed P2 shows higher antifreeze activity. The hypothermia protection assay shows that the survival rate of E.coli was 80.8 % when the concentration of peptides complexes was up to 500 μg/mL.

• Periodical: Applied Mechanics and Materials (Volume 140)
• Pages: 411-415
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.140.411
• Online since: November 2011
• Authors: Shao Yun Wang, Jun Zhao, Zhi Bin Xu, Jin Hong Wu
• Keywords: Antifreeze Peptides, Hypothermia Protection, Isolation, Preparation

References

[1] Y. Hirano, Y. Nishimiya, S. Matsumoto, M. Matsushita, S. Todo, A. Miura, Y. Komatsu, S. Tsuda, Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout, Cryobiology. 57 (2008) 46-51.

DOI: 10.1016/j.cryobiol.2008.05.006

[2] D.Q. Zhang, B. Liu, D.R. Feng, Y.M. He, J.F. Wang, Expression, purification, and antifreeze activity of carrot antifreeze protein and its mutants, Protein Expression and Purification. 35 (2004) 257-263.

DOI: 10.1016/j.pep.2004.01.019

[3] Y.J. Cai, S. Liu, X.R. Liao, Y.R. Ding, J. Sun, D.B. Zhang, Purification and partial characterization of antifreeze proteins from leaves of Ligustrum lucidum Ait, Food and Bioproducts Processing. 89 (2011) 98-102.

DOI: 10.1016/j.fbp.2010.04.002

[4] X.M. Yu, M. Griffith, Winter rye antifreeze activity increases in response to cold and drought, but not abscisic acid, Physiologia Plantarum. 112 (2001) 78-86.

DOI: 10.1034/j.1399-3054.2001.1120111.x

[5] C.A. Knight, Adding to the antifreeze agenda, Nature. 406 (2000) 249-250.

[6] S.Y. Wang, S. Damodaran, Ice-structuring peptides derived from bovine collagen, J. Agric. Food Chem. 57 (2009) 5501-5509.

DOI: 10.1021/jf900524y

[7] S.Y. Wang, K. Agyare, S. Damodaran, Optimisation of hydrolysis conditions and fractionation of peptide cryoprotectants from gelatin hydrolysate, Food Chemistry. 115 (2009) 620-630.

DOI: 10.1016/j.foodchem.2008.12.079

[8] S. Damodaran, Inhibition of ice crystal growth in ice cream mix by gelatin hydrolysate, Journal of Agricultural and Food Chemistry. 55 (2007) 10918- 10923.

DOI: 10.1021/jf0724670

[9] G.D. LV, J. Ma, Comparison of two methods for measuring the biological activity of antifreeze protein (Chinese), Journal of Xinjiang University (Natural Science Edition). 24 (2007) 77-80.