Optimization of Extraction Process of Lycopene from Watermelon (Citrullus lanatus) by Response Surface Methodology

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The extraction processing of lycopene from watermelon was investigated in this paper. Based on single factor experiments, response surface methodology (RSM) was adopted to study the key parameters such as number of extraction, extraction temperature, extraction time in order to increase extraction rate of lycopene. A second order quadratic equation was established and the applicability of model and interaction involved factors on predicting the lycopene extracting content was verified. The results indicated that the optimum extraction conditions were hexane (containing 2% dichloromethane) as extraction solvent, ratio of solvent to raw material of 3:1 (mL/g), number of extraction of 2, extraction time of 1.9 h and extraction temperature of 29.8°C. Under these conditions the extracting content of lycopene was 14.71±0.22 mg/kg, which is well in agreement with value predicted by the model.

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385-393

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November 2011

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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[1] Critiane. SM, Obdulio GM, Balbi ME, Extraction of lycopene from tomato sauce with mushrooms (Agaricus brasiliensis), determined by high-performance liquid chromatography. International Journal of Food Sciences and Nutrition, 60 (2009) 72-78.

DOI: 10.1080/09637480802090445

Google Scholar

[2] Di Mascio P, Kaiser S, Sies H. Lycopene as the most efficient biological carotenoid singlet oxygen quencher, Arch Biochem Biophys. 274 (1989) 532-538.

DOI: 10.1016/0003-9861(89)90467-0

Google Scholar

[3] Information on http://www.nal.usda.gov/fnic/foodcomp/ [2001].

Google Scholar

[4] Heinonen MI, Ollilainen V, Linkola EK, Carotenoids in finish food: vegetables, fruits, and berries. J Agric Food Chem. 37 (1989) 655-659.

DOI: 10.1021/jf00087a017

Google Scholar

[5] Tee ES and Lim L. Carotenoids composition and content of Malaysian vegetables and fruits by the AOAC and HPLC methods, Food Chem. 41 (1991) 309-339.

DOI: 10.1016/0308-8146(91)90057-u

Google Scholar

[6] Mangels AR, Holden JM, Beecher GR, Carotinoid content of fruits and vegetables: an evaluation of analytical data, AM Diet Assoc. 93 (1993) 284-296.

Google Scholar

[7] Giovanni, M. Response surface methodology and product optimization, Food Technology. 37 (1983) 41-45.

Google Scholar

[8] Gan, C. Y., Abdul Manaf, N., & Latiff, A. A. Optimization of alcohol insoluble polysaccharides (AIPS) extraction from the Parkia speciosa pod using response surface methodology (RSM). Carbohydrate Polymers.79 (2010a) 825-831.

DOI: 10.1016/j.carbpol.2009.10.006

Google Scholar

[9] Gan, C. Y., Abdul Manaf, N., & Latiff, A. A. Physico-chemical properties of alcohol precipitate pectin-like polysaccharides from Parkia speciosa pod. Food Hydrocolloids. 24 (2010b) 471-478.

DOI: 10.1016/j.foodhyd.2009.11.014

Google Scholar

[10] Ge, Y., Ni, Y., Yan, H., Chen, Y., & Cai, T.. Optimization of the supercritical fluid extraction of natural vitamin E from wheat germ using response surface methodology, Journal of Food Science. 67 (2002) 239-243.

DOI: 10.1111/j.1365-2621.2002.tb11391.x

Google Scholar

[11] Li, Q, H.,&Fu, C. L. Application of response surface methodology for extraction optimization ofgerminant pumpkin seeds protein, Food Chemistry. 92 (2005) 701-706.

DOI: 10.1016/j.foodchem.2004.08.042

Google Scholar

[12] Qiao, D. L., Kea, C L., Hua, B., Antioxidant activities of polysaccharides from Hyriopsis cumingil, Carbohydrate Polymers. 78 (2009) 199-204.

DOI: 10.1016/j.carbpol.2009.03.018

Google Scholar

[13] Wayne W. Fish, Penelope Perkins-Veazie, and Julie K. Collins. A quantitative assay for lycopene that utilizes reduced volumes of organic Solvents, Journal of food composition and analysis. 15 (2002) 309–317.

DOI: 10.1006/jfca.2002.1069

Google Scholar

[14] Guo, X., Zou, X., &Sun, M. Optimization of extraction process by response surface methodology and preliminary characterization of polysaccharides from Phellinus igniarius, Carbohydrate Polymers, 80, (2010)344-349.

DOI: 10.1016/j.carbpol.2009.11.028

Google Scholar