Optimization of DPPH Radical Scavenging Activity of Exopolysaccharides from Marine P. chrysogenum HGQ6 in Submerged Fermentation Using Response Surface Methodology

Abstract:

The objectives of this study were to investigate the effect of fermentation medium on the DPPH radical scavenging activity of exopolysaccharides from marine Penicillium chrysogenum HGQ6 by response surface methodology (RSM). A two-level fractional factorial design was used to evaluate the effect of different components of the medium. Maltose, FeSO4, and K2HPO4 were important factors significantly affecting DPPH radical scavenging activity. These selected variables were subsequently optimized using a Box-Behnken design, and response surface analysis. The optimal medium compositions were (% w/v): maltose 2.71, FeSO4 0.0016, K2HPO4 0.1, and KNO3 1.0. Under these optimal conditions, the DPPH radical scavenging rate achieved 34.0%, which agreed with the predicted values.

• Periodical: Applied Mechanics and Materials (Volume 140)
• Pages: 379-384
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.140.379
• Online since: November 2011
• Authors: Gan Lin Guo, Lei Guo
• Keywords: DPPH Radical-Scavenging Activity, Exopolysaccharides, Medium Optimization, Penicillium chrysogenum, Response Surface Method (RSM)

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