Loop-Mediated Isothermal Amplification Method for Rapid Detection of Shigella dysenteriae

Abstract:

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The study was aimed to develop a loop-mediated isothermal amplification (LAMP) method which amplifies DNA with high specificity and rapidity for the detection of Shigella dysenteriae. A set of four primers was designed for recognizing six distinct sequences on the target ipaH of S. dysenteriae. By the method, the target DNA was amplified within 1h under isothermal condition at 65 °C. The sensitivities of the LAMP for detecting pure culture and genomic DNA were 1.04 CFU/ml and 1.06 fg/μl, while the sensitivities of PCR method were 1.04×102 CFU/ml and 1.06 pg/μl. Furthermore, the LAMP assay was examined for its ability to detect S. dysenteriae in artificially contaminated lettuce sample, the detection limits of this LAMP assay and the PCR method were 4.60 CFU/g and 4.60×102 CFU/g, respectively.

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Periodical:

Edited by:

Robert Zhu

Pages:

369-373

DOI:

10.4028/www.scientific.net/AMM.140.369

Citation:

Q. P. Zhong et al., "Loop-Mediated Isothermal Amplification Method for Rapid Detection of Shigella dysenteriae", Applied Mechanics and Materials, Vol. 140, pp. 369-373, 2012

Online since:

November 2011

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Price:

$35.00

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