Optimization of the Freeze-Drying Media and Survival throughout Storage of Freeze-Dried Aerotolerant Bifidobacterium animailis Subsp. Lactis Qq08
The aim of the present work was to optimize the formula of cryoprotectants for freeze drying process of the previous selected Bifidobacterium animailis subsp.lactis Qq08 (Bl Qq08). Single factor and orthogonal experiments were conducted to optimize the cryoprotectants formula. The formula of cryoprotectants was optimized as follows: skim milk 15%, lactose 8%, Vc 1.5% and glycerol 3%. In the triplicate lyophilization tests, the average survival rate of Bl Qq08 reached 91.23%.The optimized formula of cryoprotectants was suitable for freeze drying of the Bl Qq08.
Q. Q. Li et al., "Optimization of the Freeze-Drying Media and Survival throughout Storage of Freeze-Dried Aerotolerant Bifidobacterium animailis Subsp. Lactis Qq08", Applied Mechanics and Materials, Vol. 140, pp. 394-399, 2012