Analysis of CPn0308 Immunogenicity in the Infected Patients with CPn

Abstract:

Objectives To analyze CPn0308 immunogenicity in the CPn infected patients with ICVD. Methods ①Clinical blood samples and separate plasma from blood and extract the DNA from PBMC were collected. ②The CPn IgG and CPn IgM were detected by using CPn specified diagnose kit. ③CPn DNA from PBMC was assayed by using PCR.④Imunogenicity of the endogenous CPn0308 was analyzed by Western Blotting. Results①Among the 70 patients with ICVD, the positive rate of CPn IgG was 51.43%, and it was higher than that of control; The positive rate of CPn IgM was 2.86%, and that of control was 8.47%. ②The positive rate of CPn DNA in PBMC from patients with ICVD was 81.08%，and it was higher than that of control. ③After plasma samples were diluted as 1:500, the antibodies to endogenous CPn0308 were still detected by using Western Blotting. The reaction was blocked after the plasma was pre-absorbed with GST-CPn0308 beads ahead, but not pre-absorbed with GST-CPn0186 beads. Conclusion ①There is an increase in the positive rate of CPn IgG in the patients with ICVD. ②The positive rate of CPn DNA in PBMC from the patients with ICVD is higher than that of the control. The positive rate for CPn by PCR is higher than that of EIA, and CPn DNA detection is a more sensitive method. ③The studies preliminarily demonstrate that CPn0308 has immunogenicity in the individual infected with CPn.

• Periodical: Applied Mechanics and Materials (Volume 140)
• Pages: 95-99
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.140.95
• Online since: November 2011
• Authors: Ping Li, Xiao Hui Jia, Yan Zhou, Xiao Yan Han, Yu Hong Tang, Xiao Jun Li, Tian Jun Jia
• Keywords: CPn0308, Expression Pattern, Fusion Protein, Immunogenicity, Inclusion Membrane Protein

References

[1] Jha HC, Vardhan H, Gupta R, et al. Higher incidence of persistent chronic infection of Chlamydia pneumoniae among coronary artery disease patients in India is a cause of concern, J. BMC Infectious Diseases. 7(2007) e48.

DOI: 10.1186/1471-2334-7-48

[2] Rockey DD, Scidmore MA, Bannantine JP, et al. Proteins in the chlamy- dial inclusion membrane, J. Microbes Infect. 4(2002) 333-340.

DOI: 10.1016/s1286-4579(02)01546-0

[3] Cortes C, Rzomp KA, Tvinnereim A, et al. Chlamydia pneumoniae inclusion membrane protein CPn0585 interacts with multiple Rab GTPases, J. Infect Immun. 75(2007) 5586-5596.

DOI: 10.1128/iai.01020-07

[4] Chen C, Chen D, Sharma J, et al. The hypothetical protein CT813 is localized in the Chlamydia trachomatis inclusion membrane and is immunogenic in women urogenitally infected with C. trachomatis, J. Infect Immun. 74(2006) 4826-4840.

DOI: 10.1128/iai.00081-06

[5] Diagnostic point of various cerebrovascular diseases, J. Chinese Journal of Neurology. 29(1996) 379.

[6] Jia T, Li P, Zhang S, et al. Establishment and Premilinary Application of A Method for Detection of Mutation at Site 54 of MBL EXON Ⅰ Gene, J. Chinese Journal of Biologicals. 16(2003) 327-329.

[7] Johnsen SP,Overvad K,ostergard L, et al. Chlamydia pneumoniae seropositivity and risk of ischemic stroke : a nested case-control study, J. European Journal of Epidemiology. 20(2005) 59-65.

DOI: 10.1007/s10654-004-4341-5

[8] Dowell SF, Peeling RW, Boman J, et al. Standardizing Chlamydia pneumo -niae assays: recommendations from the Centers for Disease Control and Prevention (USA) and the Laboratory Centre for Disease Control (Canada) , J. Clin Infect Dis. 33(2001) 492-503.

DOI: 10.1086/322632

[9] Littman AJ, Jackson LA, Vaughan TL. Chlamydia pneumoniae and Lung Cancer: Epidemiologic Evidence, J. Cancer Epidemiol Biomarkers Prev. 14(2005) 773-778.

DOI: 10.1158/1055-9965.epi-04-0599

[10] Kumar S, Hammerschlag MR. Acute Respiratory Infection Due to Chlamydia pneumoniae: Current Status of Diagnostic Methods, J. Clinical Infectious Diseases. 44(2007) 568-576.

DOI: 10.1086/511076