Hydrolysis of Corncob Residue by Enzymes from Fungi C1067 and the Products Analysis by Capillary Electrophoresis

Abstract:

To achieve high enzymatic hydrolytic efficiency of corncob residue, one strain, Trichoderma C1067, with the enzyme activity of corncob residue as high as 4.5g/L glucose liberated per hour in 0.5mL crude cellulase and 0.5mL citric acid /sodium citrate buffer under the assay conditions, was carefully studied. The optimal carbon and nitrogen sources used in the fermentation medium are as follows: corncob residues 2.0%, wheat bran 1.5%, corncob 0.5%, rice straw 0.5%, peptone 0.1% and corn steep liquor 4%. The optimal conditions of saccharification for corncob are 55°C and pH 4.0. The sugars gained in the hydrolysate consist of glucose 4.1% and xylose 0.4% after enzymolysis for 72h, tested by capillary electrophoresis.

• Periodical: Applied Mechanics and Materials (Volume 145)
• Pages: 68-72
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.145.68
• Online since: December 2011
• Authors: Jian Lu Ma, Xiao Min Fang, Xin Jiong Guo, Huan Fei Liu, Gui Fu Dai, Jian Wu
• Keywords: Capillary Electrophoresis, Cellulase, Corncob Residue, Enzymolysis

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