Research on Fluorescence Imaging Detecting System of Intracellular Calcium

Abstract:

A new effective imaging detecting system has been used to determinate of intracellular calcium. The fluorescence Image acquisition and analysis was realized with fluorescence imaging detecting system. A designed microfluidic chip has been built. Cell fostering, staining and other operations can be implemented on it. Based on it, a new detecting means was developed for fluorometric measurement. The concentration of intracellular calcium ion can been calculated by using the fluorescence ratio method. A series of experiments were performed by using the detecting system. The experimental results show the ratio of fluorescence intensity was positively correlated to the raising temperature and enhancing shear stress.

• Periodical: Applied Mechanics and Materials (Volumes 148-149)
• Pages: 38-41
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.148-149.38
• Online since: December 2011
• Authors: Bao Liu, Si Xiang Zhang, Wei Zhou
• Keywords: Cell Detection, Concentration of Calcium Ion, Fluorescence Intensity, Fluorescence Ratio, Imaging Detecting, Microfluidic Chip, Shear Stress

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