Characterization and Immunological Evaluation of Chitosan Nanoparticles as Adjuvants for Bovine Coronavirus N Protein

Abstract:

Chitosan nanoparticles (CNP) loaded with nucleocapsid protein of bovine coronavirus (BCV N) were prepared by ionic cross-linking method using sodium tripolyphosphate (TPP) as cross-linking agent. CNP loaded with BCV N protein was intramuscularly administered into Balb/c mice. Serum levels of anti-N protein IgG, IgM, and IgA antibodies were dynamically monitored by indirect ELISA method. Results showed that in the BCV N-loaded chitosan group, both IgA and IgG levels were found to increase significantly after the second immunization comparable to those of the Montanide ISA 206 groups. The IgM content in serum increased after the second immunization in the CNP loaded BCV N protein group. These findings indicate that CNP can be used as adjuvant in veterinary vaccine field.

• Periodical: Applied Mechanics and Materials (Volume 161)
• Pages: 113-120
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.161.113
• Online since: March 2012
• Authors: Qing Shen Sun, Jia Ling Zhang, De Quan Han, Yong Bo Yang, Li Zhu, Li Yu
• Keywords: Bovine Coronavirus N Protein, Chitosan Nanoparticles, Sustained Release

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