Synthesis of Gold Nanoparticles by Methanobactin

Abstract:

Methanotrophic physiology is strongly affected by the amount of bioavailable copper, which regulates the enzymes used by methanotrophs to oxidize methane. Methanobactin is a small peptide secreted by methanotrophic bacteria that binds copper outside of the bacterial cell. In this study, gold nanoparticles were firstly synthesized with citrate acid, followed by addition of methanobactin. Then as CuSO₄ was continuously added to the solution, we found a red shift in the Uv-vis absorption spectra. Meanwhile, for the mixture of methanobactin, 1,4-resorcinol and chlorauric acid, we also found an absorption at 540nm after 1h in dark. We presume that methanobactin has the bioactivity of protecting gold nanoparticles as well as delivering electron for the catalytic synthesis of gold nanoparticles. These molecules then go on to form gold nanoparticles. Gold nanoparticles can be seen with the naked eye, as they turn a gold solution from yellow/gold to a deep cranberry/red. The nanoparticles also display a characteristic absorbance of approximately 540 nm on absorption spectra.

• Periodical: Applied Mechanics and Materials (Volumes 182-183)
• Pages: 245-248
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.182-183.245
• Online since: June 2012
• Authors: Tie Nan Zhang, Jia Ying Xin, Xiu Feng Zhang, Ying Xin Zhang, Chun Gu Xia
• Keywords: 1,4-Resorcinol, Gold Nanoparticles (GNP), Methanobactin, Methylosinus trichosporium 3011, UV–vis Absorption Spectra

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