Purification and Characterization of an Alkaline Protease from Bacillus alcalophilus

Shan Shan Liu; Li Li Wang; Lin Yuan

doi:10.4028/www.scientific.net/AMM.192.285

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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 192Purification and Characterization of an Alkaline...

Purification and Characterization of an Alkaline Protease from Bacillus alcalophilus

Abstract:

The purification and characterization of an alkaline protease produced by Bacillus alcalophilus were investigated. The enzyme was purified in two steps: concentrating the crude enzyme using ammonium sulfate precipitation, followed by cation-exchange chromatography. The purified protease had a molecular mass of approximately 28 kDa, was highly active over an alkaline pH range of 10.0 to 11.0, and remained stable over a pH range of 7.0 to 12.0. The optimum temperature for the enzyme activity was found to be 40~60°C, while the thermotolerance of the enzyme was poor. Therefore, these characteristics of the protease indicate its potential for a wide range of commercial applications.

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Periodical:

Applied Mechanics and Materials (Volume 192)

Pages:

285-288

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.192.285

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Online since:

July 2012

Authors:

Shan Shan Liu, Li Li Wang, Lin Yuan

Keywords:

Alkaline Protease, Bacillus alcalophilus, Enzyme Characterization, Enzyme Purification

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