Isolation, Screening and Identification of Phenol-Degrading Bacteria from Coking Wastewater

Abstract:

Phenol is the most common pollutant which can be found in several types of industries. It is highly toxic to human beings. To seek the best phenol-degrading bacteria, we collected activated sludge from an aeration tank of the coking wastewater treatment plant, Benxi Iron and Steel Corporation. Five phenol-degrading strains, designated BS3, BS4, BS23, BS28 and BS29, were isolated and screened from activated sludge. Under the conditions of initial phenol 500 mg•L-1,170 rpm and 28°C, the removal efficiencies of BS3, BS4, BS23, BS28 and BS29 strains reached to 79.6%±1.8%, 55.2%±1.0%, 62.4%±2.6%, 78.6%±2.0% and 61.2%±1.9% within 24 h, respectively. By a series of morphological and biochemical and physiological tests, the five phenol-degrading bacteria were identified. The results indicated that they were Pseudomonas spp.. Hence these strains can be effectively used for bioremediation of phenol contaminated water.

• Periodical: Applied Mechanics and Materials (Volumes 209-211)
• Pages: 2027-2031
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.209-211.2027
• Online since: October 2012
• Authors: Xue Kai Sun, Xi Ping Ma, Cheng Bin Xu, Jie Bai, Wei Zhang
• Keywords: Coking Wastewater, Identification, Phenol, Pseudomonas Spp., Screening

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