Separation of Curcumine (a Functional Food Colourant Material) from Rhizoma curcuma longae by Aqueous Two-Phase Systems

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The objective of this paper was to separate curcumine from Rhizoma curcuma longae and aqueous two-phase extraction technology was employed to perform the study. Four types of PEG (400, 600, 2000 and 4000) and three types of salt ((NH4)2SO4, KH2PO4 and K2HPO4) were tested to form the aqueous two-phase systems. The results showed that the ATPS composed of PEG 400 and (NH4)2SO4 were the best. And the partition coefficient of curcumine could reach 21.79 when the concentration of PEG 400 and (NH4)2SO4 was 14.6% and 26% respectively.

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961-964

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November 2012

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© 2012 Trans Tech Publications Ltd. All Rights Reserved

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